新型仿生膜体系的制备及其应用研究

项目名称： 新型仿生膜体系的制备及其应用研究

项目编号： No.21273059

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 韩晓军

作者单位： 哈尔滨工业大学

项目金额： 80万元

中文摘要： 仿生膜作为生物膜的模型被广泛用于研究生物膜的各种生物物理性质。本课题针对此领域目前存在的难点、热点问题，以及其发展趋势，利用化学、物理、生物、材料、微流控等学科的知识，研究以下内容：1）利用微流体技术制备内外腔溶液可控、尺寸可控、分布均一的巨型磷脂泡囊，并将其用于长链DNA的扩增研究；2）利用液滴和自组装等技术在电极表面制备高阻抗仿生膜体系，并将其用于离子通道研究；3）制备可同时进行荧光显微和电化学技术表征的仿生膜体系，并用其研究膜电势对膜流动性的影响，膜的流动性对离子载体和氧化还原蛋白质行为的影响，以及用其对霍乱毒素进行高灵明检测。通过这些研究的开展将丰富仿生膜自组装体系的内容，增强对生物膜结构、性质和功能的认识，拓展其在生物检测上的应用领域。

中文关键词： 磷脂双层膜；微流控；巨型磷脂泡囊；离子通道；自组装膜

英文摘要： Lipid bilayer membranes, as biomemebrane models, have been widely used to study all kinds of biophyisical properties of biomembrane. This project targets the key problems of the lipid bilayer research field, and contains following subprojects. Firstly, the uniform size giant unilamellar vesicles(GUV) will be fabricated using microfluidic devices. The inside/outside solution of GUV can be controlled during fabricating process. The size of GUV is also tunable. The GUV will be used as microreactor to do polymerase chain reaction (PRC) of long chain DNA. Secondly, a supported lipid bilayer membrane with high electric resistance will be developed on electrode surface using droplet and self-assembling techneques. It will be used to measure ion channel activeties. Thirdly, a surpported bilayer lipid membrane suitable for electrochemical and fluorescent simutanous detection will be fabricated on indium tin oxide (ITO) electrode surfaces. The influence of membrane potential on membrane mobility will be studied. The influence of membrane mobility on the function of ionphore and redox protein will be investigated as well. It also can be used to detect cholear toxin with high sensitivity due to the simutanious use of two techniques. The outcomes of this project will enrich the type of artificial membrane, and enhance the un

英文关键词： Lipid bilayer membrane；Microfluidics；Giant unilamellar vesicles；Ion channel；Self-assembled monolayer