纳米界面蛋白质分子作用机制研究

项目名称： 纳米界面蛋白质分子作用机制研究

项目编号： No.21303043

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 王公轲

作者单位： 河南师范大学

项目金额： 25万元

中文摘要： 纳米界面蛋白质分子作用机制的研究是一个涉及物理、化学、生物、医学和材料科学等相关学科的热点课题。本项目拟在不同尺寸和不同表面修饰基团的纳米金调控合成的基础上,利用等温滴定量热（ITC）、紫外-可见吸收光谱、荧光光谱等宏观方法与圆二色谱（CD）、动态光散射(DLS)、扫面电镜（SEM）等微观技术手段相结合，系统地研究纳米界面上纳米粒子与蛋白质相互作用的热力学驱动因素和作用机制，从而建立它们之间相互作用的热力学模型。探讨纳米金尺寸和表面修饰基团对它们之间相互作用性质的影响，分析纳米界面蛋白质构象和纳米金聚集状态的变化及其影响因素，并从热力学角度确定引起这些变化的可能因果关系，为纳米粒子在生物和医学领域的功能化和安全应用提供科学依据。

中文关键词： 纳米界面；蛋白质；相互作用；热力学；

英文摘要： The interaction mechanism of nanoparticles with proteins in nano-interfaces is a very hot topic in multi-disciplinary areas, such as physics, chemistry, biology, medicine and material science. In this project, based on the synthesis of gold nanoparticles with different sizes and different modification groups, the binding mechanism and the thermodynamic driving factors for the interaction of nanoparticles with proteins in nano-interfaces are investigated by combined macroscopic (ITC, UV-Vis absorption, fluorescence spectroscopy) and microscopic (CD, DLS, SEM) methods. On the basis of these results, we will build a thermodynamic model for the interaction of gold nanoparticles with proteins. The effects of size and modification groups of gold nanoparticles on the interaction are examined in detail. The changes of protein conformation and gold nanoparticles aggregation in nano-interfaces, as well as the influence factors of these changes are analyzed. Furthermore, the potential cause-effect relationships of the microstructure changes are also determined from the viewpoint of thermodynamics. These results will provide some new insights into the functionalization and safe application of nanoparticles in biological and medical areas.

英文关键词： Nano-interfaces；Protein；Interaction；Thermodynamics；