"三明治"新结构近红外上转换近紫外光纳米粒子构建及其癌症诊断和可视化治疗方法研究

项目名称： "三明治"新结构近红外上转换近紫外光纳米粒子构建及其癌症诊断和可视化治疗方法研究

项目编号： No.61275202

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 孔祥贵

作者单位： 中国科学院长春光学精密机械与物理研究所

项目金额： 90万元

中文摘要： 针对癌症难于早期诊断和准确治疗的重大问题，本项目基于生物光谱窗口内的近红外光上转换高能量可见光或近红外光生物标记可视化功能和近紫外光对癌细胞诱导凋亡机理以及抗体生物靶向功能，设计和构筑"三明治"多功能壳核新结构上转换近紫外发光纳米晶("S"UNCs)，获得用于癌症治疗的高效上转换近紫外发光纳米平台。研究提高"S"UNCs上转换近紫外发光效率的新途径，获得高效上转换近紫外光的"S"UNCs；研究"S"UNCs与抗体生物"柔性"仿生组装技术，获得"S"UNCs靶向癌细胞的高灵敏性和特异性；研究"S"UNCs上转换紫外发光与体外癌细胞相互作用和动力学及其鼠体癌症靶向诊断和可视化治疗效应，建立近红外光上转换近紫外发光癌症早期诊断和可视化治疗一体化的创新方法。开展上述重要问题的研究，将为发展癌症早期诊断和可视化治疗一体化新方法提供研究基础和科学依据。

中文关键词： 三明治纳米新结构；上转换蓝紫发光；上转换发光生物成像；癌症可视化诊断；诊断和可视化治疗

英文摘要： Aiming at the challenging problems of the early diagnosis and therapy of cancers, we base on the mechanism of cancer cell death induced by ultraviolet light and the function of cancer cells labeled using near infraredupconversion luminescence from the novel upconversion nanocrystalcoupled withantibody under exitation of near infrared light with 980nm, the novel upconversion crystals with multiple shells transferring near infrared to near ultraviolet are devised and constructed. To study the novel ways of enhancing the up-converting efficiency of near infrared to ultraviolet from the"S"UNCs whichare used in cancer diagnosis and visualization therapy with very high effeciency; To study the assembly technologies of coupling "S"UNCs wity antibody in soft way to increase the biodetecting sensitivity and specific. Besides, the interactions and dynamics between "S"UNCs and antibodies will be studied, including effects of visualization therapy of cancers in vivo, to get the innovating methods of visualizationtherapyof cancerunder guiding by near infrared upconvertion light, based on the ultraviolet light with high energy. To study these challenging problems mentioned above is with an important signification and to establish some fundaments for devolping a novel technologies of early diagnosis and therapy via an unconver

英文关键词： "sandwich" nanostructures；upconverting blue and ultraviolet light；upconversion luminescence bioimaging；highly precise visualization diagnosis of cancer；integration of diagnosis and visualization therap