金纳米颗粒比色分析新技术与肺癌基因目视诊断新方法研究

项目名称： 金纳米颗粒比色分析新技术与肺癌基因目视诊断新方法研究

项目编号： No.21275002

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 吴再生

作者单位： 福州大学

项目金额： 80万元

中文摘要： 研究了表面多位点牵引效应DNA衍生纳米金复合物合成路线与光学、电化学、团聚行为等性质，发展了电化学活性探针表面限定多位点牵引(EPSP)信号放大策略；探索了EPSP电化学传感界面构建路线；整合出一种灵敏度高、选择性好、适合于p53点突变准确识别的电化学传感技术，并初步运用于肺癌诊断。 发表论文12篇（总影响因子70左右），联合培养博士硕士研究生8名，培养省级、国际基金专项人才各3人次。 本项目根据纳米金（GNP）团聚发生颜色变化现象，拟通过利用GNP团聚新机制，发展一种无需任何检测仪器、容易操作、便于普及推广的p53基因目视识别技术，直接应对我国人口总数多、人口流动性大、经济不发达、肿瘤发病率与死亡率高的情况与目前肺癌临床诊断费用高、分析程序繁杂的问题，以期实现对肿瘤早期诊断、及时治疗、准确分期、疗效监测与预后评估等做出贡献，为肺癌诊治与高危人群定期筛查或普查提供可靠的数据支持与方法指导。

中文关键词： 恶性肿瘤；基因诊断；目视比色检测；升级化复合型滚环扩增；核酸纳米器件

英文摘要： The method to synthesize DNA-modified gold nanoparticles that can be pulled onto the substrate surface via several connecting points has been studied, and the optical, electrochemical and aggregation behavior have been investigated. The strategy to amplify the signal based on electrochemical active probes pulled onto the surface via several connecting points (EPSP) has been developed, and the corresponding sensing interface has been characterized. The electrochemical sensing technology for the detection of p53 mutant gene has been fabricated, and high detection sensitivity and selectivity have been achieved. The applicability of this sensing system for the diagnosis of lung cancer has been primarily confirmed. These achievements indicate the accomplishment of the expected objectives of the study. Twelve research papers have been published by the international academic journals, and the total number of impact factor is about 70. The cultivation of Master (5) and Ph.D. (3) has been accomplished on the basis of this research project, as well as other projects. Three scientific talents have been trained who had been awarded science foundation by Hunan Society of Chemistry and Chemical Industry, the Education Department in Hunan province and McMaster University. On the basis of gold nanoparticles (GNP) aggregation-

英文关键词： Malignant tumor；gene diagnosis；visual colorimetric detection；enhanced complex rolling-circle amplification；DNA nanostructures