生理活性物质的电化学发光分析新方法研究

项目名称： 生理活性物质的电化学发光分析新方法研究

项目编号： No.21475149

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 张美宁

作者单位： 中国人民大学

项目金额： 80万元

中文摘要： )：大脑内的化学物质的分析检测对揭示脑功能分子机制非常重要，但是由于脑内化学和生理环境的复杂性目前对这些物质的分析仍然存在很多挑战。本项目针对这些化学物质活体在线分析方法检测信号单一，检测的物质种类有限、灵敏度不够等一些至关重要的科学问题，开展系统而深入的电化学发光分析生理活性物质的研究。拟解决的瓶颈问题包括：系统地研究生理活性物质和经典电化学发光体系的关系，探索其一般的规律和机理；设计基于酶或者智能配体的电化学发光法选择性识别生理活性物质的方法；通过研究纳米材料本身的电化学和电化学发光性质，在电极上构筑三维结构，提高电化学发光分析方法的灵敏度。可以预期，这些关键性问题的深入研究可以提出和建立6-9 种电化学发光分析新方法，这些新方法为进一步生理活性物质活体电化学发光分析研究及应用提供了前提并奠定了坚实的基础。

中文关键词： 电化学发光；生理活性物质；选择性；灵敏度

英文摘要： Quantitative in vivo monitoring of the levels of neurochemicals in extracellular fluid is of great importance to understanding the molecular basis of brain functions.However, the chemical and physiological complexity of the central nervous system makes the development of analytical methods for such a purpose very challenging. The present on-line methods for probing chemical essences in brain have some key chanlleges,such as output signal and detected biological compounds are limited, the sensitivity is not high enough. To address these problems, this project would develop some major strategies, such as study the relationship between neurochemicals and tyical ECL system to expore reaction mechanism, design ECL selective biosensor based on enzyme and aptamer,constructing 3D electrode interface with nanomaterial to futher improve the sensitvity of ECL method.It is expect to develop 6-9 ECL methods in this project, which would help to understand the physiological and pathological processes associated neurochemicals.

英文关键词： electrogenerated chemiluminescence;neurochemicals;selectivity;sensitivity