蒲黄生行熟止的物质基础及质量评价

项目名称： 蒲黄生行熟止的物质基础及质量评价

项目编号： No.81470176

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 刘晓秋

作者单位： 沈阳药科大学

项目金额： 71万元

中文摘要： 本草记载蒲黄生行熟止，现已被临床公认，即蒲黄行血，炒炭止血。一般认为蒲黄黄酮类有止血作用，但蒲黄炭黄酮类明显下降，而止血作用增强。这种药效物质基础不明确，导致质量控制没有得到根本解决。我们研究表明，蒲黄炭中酚酸类变化不明显，黄酮类变化显著，酚酸类延长凝血时间，黄酮类缩短凝血时间。当黄酮/酚酸比例减低，则凝血作用增强。因此，本课题首次提出了基于药物-药物相互作用理论研究中药药效物质基础的科学假说，探讨黄酮与酚酸不同配比在Caco2细胞吸收、血浆蛋白结合率、CYP450酶抑制作用的机制，考察酚酸类与黄酮类成分配比的药动学-药效学相关性，构建适合生行熟止的蒲黄及蒲黄炭质量评价策略。通过应用药物代谢学、酶学和分离分析等技术，系统地探讨蒲黄生行熟止的物质基础。本课题可能发现治疗血瘀证及出血证新的组分配比，为指导临床准确用药提供有力的科学证据，并为同类中药的研究提供新的模式和借鉴。

中文关键词： 蒲黄；蒲黄炭；生熟异治；药效物质；质量评价

英文摘要： The opinion of Sheng Xing Shu Zhi for Typhae Pollen was recorded in books on Chinese materia medica and has been validated by clinical. Generally，flavonoids in Typhae Pollen are considered to have hemostasis effect. But contents of flavonoids in Typhae Pollen carbonized are significantly lower, while its hemostasis effect was significantly enhanced. The unclear effective substances lead to quality control has not been fundamentally resolved yet. Our research shows that phenolic acids did not change significantly and flavonoids changes significantly in Typhae Pollen carbonized. Phenolic acids prolong hemostaisis time, and flavonoids shorten the hemostasis time. Hemostasis effect was enhanced with reducing the ratios of flavonoids/phenolic acids. Therefore, this project is first proposed research ideas on substances foundation based on the theory of drug-drug interaction. We will explore mechanism of the different proportions of phenolic acids and flavonoids in Caco2 cells absorption, plasma protein binding, and CYP450 enzyme inhibition, investigate correlation between pharmacokinetic and pharmacodynamic, find out substances foundation and build quality evaluation strategy about Sheng Xing Shu Zhi of Typhae Pollen by molecular biology, enzymology and separation and analysis techniques. And the research will find new constituents in treatment of blood stasis syndrome and hemorrhagic syndrome, and provide strong scientific evidence for guiding using of drugs accurately in clinical, and also provide a new model and reference for the research of similar traditional Chinese medicine.

英文关键词： Typhae Pollen;Typhae Pollen carbonized;different functions of prccessed products and the raw;effective substances;quality evaluation