EGFR在人外泌汗腺三维模型构建中的作用及机制

项目名称： EGFR在人外泌汗腺三维模型构建中的作用及机制

项目编号： No.81471882

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李海红

作者单位： 汕头大学

项目金额： 72万元

中文摘要： 汗腺的重建对于恢复大面积烧伤引起的泌汗功能降低有重要意义。三维培养模式（3D）是与生理相关的培养模式，它捕获了建立组织形态发生的动态和交互的信号。体外单层培养技术、皮肤标本检测和动物实验研究发现EGFR在汗腺发育、功能维持和汗腺肿瘤的发生中起着重要作用。我们利用细胞外基质成功地构建了汗腺细胞体内外3D培养模型，同时在预实验中发现：用EGFR抑制剂后，汗腺细胞不能重构3D结构。基于上述结果我们提出EGFR是细胞外基质诱导汗腺细胞重构汗腺三维结构的核心成份。EGFR活化后，又激活下游的信号通路，引起基因转录，细胞增殖、分化、凋亡和极性，从而重构汗腺三维结构的假说，本项目拟应用免疫组织化学、RT-PCR、western、免疫共沉淀、基因转染、RNAi、RNAi补救实验等手段和方法研究EGFR在汗腺3D模型构建中的作用及机制，其结果将为汗腺的修复和再生提供未来发展的新模型、新策略和基准数据。

中文关键词： 表皮生长因子受体；外泌汗腺；三维培养模型；细胞外基质；matrigel基底膜基质

英文摘要： For patients with extensive deep burns, the reconstruction of eccrine sweat gland to restore the sudomotor function is very important. Three-dimensional culture models are physiological correlated culture modes, which recapitulate the dynamic and interactive signaling in tissue morphogenesis. The in vitro monolayer culture technique, skin specimen detection and animal experiment showed that EGFR played key roles in the development, function maintain and the tumorigenesis of sweat gland. We have successfully constructed the in vivo and in vitro 3D culture model of sweat glands by culturing sweat gland cell in extracellular matrix. Our preliminary studies show that the EGFR inhibitor inhibits the formation of the 3D structure. On these above grounds, we raise the hypothesis that EGFR is the central element in the ECM-induced construction of three-dimensional sweat gland models. The extracellular signals activate EGFR and then EGFR activate the downstream signaling pathway, which result in gene transcription, cell proliferation, differentiation, apoptosis and ploarity and then the construction of the 3D structure of sweat glands. The research is to study on the roles and mechanisms of EGFR in the construction of three-dimensional culture models of human eccrine sweat gland by immunohistochemistry, RT-PCR, western, co-immunoprecipitation, gene transfection, RNAi, RNAi rescue experiment, and so on. The results will provide new strategies and models as well as the baseline data on future developments of eccrine sweat gland repair and regeneration.

英文关键词： EGFR;eccrine sweat gland;three-dimensional culture model;extracellular matrix;Matrigel basement membrane matrix