NaCl胁迫下多倍体西瓜离子吸收及DNA甲基化调控机制研究

项目名称： NaCl胁迫下多倍体西瓜离子吸收及DNA甲基化调控机制研究

项目编号： No.31471893

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 刘文革

作者单位： 中国农业科学院郑州果树研究所

项目金额： 85万元

中文摘要： 土壤盐碱化和次生盐渍化是影响西瓜生产的一个重要因素，严重制约着我国西瓜优质高效可持续发展。申请人发现多倍体西瓜较其同基因型二倍体耐盐，但多倍体西瓜耐盐机理尚不明确。本项目拟以同基因型的二倍体、三倍体、四倍体西瓜和由四倍体回复突变形成的二倍体为材料，采用不同倍性西瓜互做砧木进行嫁接的方法，研究NaCl胁迫后不同类型西瓜植株各组织Na+、K+含量及离子吸收的变化，确定不同倍性西瓜盐胁迫离子吸收类型差异；并结合MSAP技术研究DNA甲基化水平和甲基化模式的变化，在MSAP基础上筛选与倍性相关的耐盐基因，并对重要的耐盐基因进行实时荧光定量分析，研究DNA甲基化调控水平上不同倍性西瓜耐盐基因的差异表达。研究结果将从离子吸收和表观遗传学方面解析多倍体西瓜的耐盐优势机理，为多倍体西瓜育种和抗逆育种提供理论依据。

中文关键词： NaCl胁迫；多倍体；西瓜；离子吸收；DNA甲基化

英文摘要： The production of watermelon is seriously affected by soil salinization and secondary salinization which restrict the quality and efficiency of sustainable development of watermelon in China. Our previous study found that the polyploid watermelon including triploid and tetraploid had better salt tolerance than its isogonics diploid, but the mechanism of salt resistance of polyploid watermelon is not clear. In this research, the different ploidy watermelon include the same genotype diploid, triploid, tetraploid and the diploid which is reverse mutation formed by tetraploid will be used as materials. The grafting interaction methods will be studied using different ploidy watermelon as rootstock, the Na+, K+ contents and ion absorption changes in different organizations of each watermelon plants after NaCl stress will be measured, and the ion absorption type of salt stress of the different ploidy watermelon will be determined; the level of DNA methylation and the change of methylation patterns using MSAP technology will be researched, the related genes of polyploidy salt tolerant based on MSAP will be screened, and the important genes in real-time fluorescence quantitative and differential expression of salt tolerance genes regulation of DNA methylation in different ploidy watermelon will be analyzed. The salt tolerance advantage mechanism of polyploidy watermelon from the aspects of ion absorption and epigenetics under salt stress will be carried out. This study will provide theoretical basis and some useful information for polyploid breeding and resistance breeding of watermelon.

英文关键词： NaCl stress;polyploid;watermelon;ion adsorption;DNA methylation