DNA甲基化和组蛋白乙酰化对高山离子芥抗冻性的调控机制研究

项目名称： DNA甲基化和组蛋白乙酰化对高山离子芥抗冻性的调控机制研究

项目编号： No.31200298

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 生态学

项目作者： 宋渊

作者单位： 兰州大学

项目金额： 28万元

中文摘要： 环境压力下，植物基因组会产生复杂的DNA甲基化和组蛋白乙酰化变化，这是一种适应性调节机制。高山离子芥作为典型的高山冰缘植物, 具有很强的抗寒能力, 是研究植物耐受冷冻环境机制的理想材料。本项目首次从表观遗传学的角度探讨以高山离子芥为代表的高山冰缘植物所特有的抗冻机制。采用MSAP甲基化敏感扩增多态性技术和生物统计学，分析冷冻胁迫下DNA甲基化修饰模式的变化和规律；利用生物信息学对DNA甲基化调控的基因以及参与的通路进行整理；结合蛋白质差异表达筛选高山离子芥在低温胁迫下受DNA甲基化调控的冷响应基因；利用ChIP技术和重亚硫酸盐测序分析在冷冻胁迫下靶基因启动子和第一外显子区的组蛋白乙酰化、核小体结合和胞嘧啶甲基化变化情况。本项目旨在发现冰缘植物在低温环境下的表观遗传修饰调控规律，为揭示高山冰缘植物特有的抗冻分子机制提供新的视角，为制定新的农作物抗冻策略提供理论支撑。

中文关键词： 表观遗传修饰；高山离子芥；植物抗冻性；植物乙醇脱氢酶；调控机制

英文摘要： Under environmental stress, plant genome adopts complex changes in DNA methylation and histone acetylation as a mechanism of adaption to the stress. As a prototypic periglacial plant，Chorispora bungeana Fisch&C.A.Mey is of great capability on anti-freezing. It is an ideal material on research how plants adapt to low temperature, chilling and freezing environment. Our project aims to discuss the antifreeze mechanism in Chorispora bungeana from new view of epigenetics. The DNA methylation states and alterations under cold stress will be examined by MSAP (methylation sensitive amplification polymorphism) and biostatistics. We will identify candidate genes and pathways regulated by DNA methylation through bioinformatics methods. Combined with protein two-dimensional electrophoresis (2-DE) data, we expect to find genes that are regulated by DNA methylation and also respond to cold stress. The changes on the histone acetylation pattern, the situation of nucleosome integrating and the changes of cytocine methylation at the promoter and first exon regions of the target genes under cold stress will be analyzed by ChIP (chromatin immunoprecipitation) and bisulfite genomic sequencing. This project aims to find the regulation of epigenetic changes under cold stress in periglacial plants. It provides a new view on molecular

英文关键词： Epigenetic modifications；Chorispora bungeana；Anti-freezing；Plant alcohol dehydrogenase；Regulation mechanism