与心房颤动相关的HAND1等转录因子基因突变鉴定及功能研究

项目名称： 与心房颤动相关的HAND1等转录因子基因突变鉴定及功能研究

项目编号： No.81500246

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 李新

作者单位： 首都医科大学

项目金额： 18万元

中文摘要： 心房颤动是临床最常见的心律失常，严重危害人民健康。国外全基因组关联研究提示参与心脏和心血管发育的转录因子与房颤有关，国内缺乏系统性易感基因研究。我们前期发现HAND1是中国人孤立性房颤的新关联基因，并推测HAND1等转录因子可通过调控心肌纤维化或离子通道而影响房颤发生发展。本研究拟选取20例房颤伴高度心房纤维化的病例，借助二代高通量捕获测序技术全面筛查多个心脏发育转录因子基因的功能变异，深入探讨转录因子影响房颤发生发展的机制和它们之间的相互作用网络。对二代测序发现的候选变异在300：300的病例对照样本中验证其与房颤和心房纤维化的相关性。选择一个经过验证与房颤显著相关且推测有强致病性的转录因子突变构建点突变转基因小鼠模型，开展机体水平、组织水平和细胞水平的研究以确定其致房颤发生发展的机制。本研究结果对于未来开展房颤个体化医疗、降低房颤危害和新药开发具有重要意义。

中文关键词： 心房颤动；转录因子；遗传变异；心肌纤维化

英文摘要： Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia. Genome-wide association studies (GWAS) and our own candidate gene study have highlighted the role of cardiac transcriptional factors (TFs) in AF. We identified HAND1 as a new AF-associated TF gene. According to our results from in silico analysis, we hypothesize that HAND1 and related TFs may involve in AF through regulation of atrial fibrosis. To test the hypothesis, we propose a delicated design used next-generation sequencing technology to sequence 20 carefully selected AF patients with a rapid progress of atrial fibrosis to idtentify founctional variants. The identified variants will be further validated in 300 unrelated patients with AF and 300 healthy controls. Subgroup analysis according to atrial fibrosis progress will also be performed to test the association between variants and atrial fibrosis. At last, a replicated TF variant with predicted pathogeneity will be explored in a transgenic mouse model to test pathogenity and its functional mechenism in the progress of AF and/or atrial fibrosis. Our results will improve the genetic understanding of AF, which will pave the way to personalized medication of AF and is also helpful in new antiarrhythmia drug development.

英文关键词： Atrial fibrillation;Transcript factors;Genetic variants;Cardiac fibrosis