深海细菌新型M12家族弹性蛋白酶myroilysin对胶原蛋白的膨胀机制与应用

项目名称： 深海细菌新型M12家族弹性蛋白酶myroilysin对胶原蛋白的膨胀机制与应用

项目编号： No.41276149

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 天文学、地球科学

项目作者： 陈秀兰

作者单位： 山东大学

项目金额： 81万元

中文摘要： 深海沉积物中存在着大量产蛋白酶细菌，对深海蛋白酶资源的研究和开发可以发现具有新的功能和用途的蛋白酶。我们前期研究发现深海细菌Myroides profundi D25分泌的M12家族新型弹性蛋白酶myroilysin对胶原蛋白有很强的膨胀作用，但不降解胶原蛋白，预示着该酶是一种很好的胶原蛋白改性剂。本项目将在前期研究的基础上首先研究myroilysin膨胀胶原蛋白的机制，通过研究myroilysin处理对胶原蛋白腱、纤维束、纤丝和胶原分子等不同级别结构的影响和分析myroilysin的解旋酶活性、对胶原蛋白中的糖蛋白和蛋白聚糖等成分的降解活性等，阐明myroilysin膨胀胶原蛋白的机制。然后利用myroilysin能膨胀胶原蛋白的特性制备真皮胶原基质支架，并通过一系列的体外体内实验分析支架的性能，评价myroilysin在胶原基质支架制备中的应用潜力。从而开发深海新型细菌蛋白酶的用途。

中文关键词： 海洋细菌；蛋白酶；无细胞真皮基质；支架；组织工程

英文摘要： There are a lot of protease-producing bacteria in deep-sea sediment. Research and development on the bacterial proteases from deep-sea sediment is helpful to find novel proteases with new function and use. Our previous study showed that the M12 novel elastinase myroilysin secreted by the deep-sea bacterium Myroides profundi D25 has strong collagen-swelling ability, indicating that it may be a good modifier of collagen. Based on the previous results, this program will first study the mechanism of myroilysin to swell collagen. The effect of myroilysin on the structures of tendon, fascicle, fibril and molecular of collagen, the unwinding activity of myroilysin to collagen molecular, the degradation activity of myroilysin to the proteoglycans and glycoproteins in collagen will be studied to elucidate the collagen-swelling mechanism of myroilysin. Then, myroilysin will be used to prepare dermal collagen scaffolds by means of the collagen-swelling ability of myroilysin. In vitro and in vivo experiments will be performed to analyze the property of the scaffolds.The potential of myroilysin in collagen scaffold preparation will be evaluated. So this program will explore the use of novel protease form deep-sea bacteria.

英文关键词： marine bacteria；protease；acacellular dermal matrix；scaffold；tissue engineering