不同酵母Hap4p调控网络的研究

项目名称： 不同酵母Hap4p调控网络的研究

项目编号： No.31300055

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 郭晓贤

作者单位： 湖南科技学院

项目金额： 23万元

中文摘要： 有氧条件下真核生物主要通过在线粒体中的呼吸途径利用葡萄糖进行能量代谢，而Crabtree-positive酵母在葡萄糖存在的环境下则进行有氧发酵。有氧发酵的进化与转录重排关系密切。Saccharomyces cerevisiae 中HAP转录复合体调控着发酵-呼吸系统的平衡，其中的转录因子Hap4p在非发酵性培养基中被诱导表达。在不同的酵母中Hap4p仅在氮端与碳端分别存在一个短的保守模体，而序列的其它部分则有很大的差异。本课题以Pre-WGD 及Post-WGD 酵母中参与细胞产能过程调控的HAP复合体作为研究对象，在不同培养条件下研究其中起调控作用的Hap4p 的表达模式，使用染色体免疫沉淀结合大规模高通量测序技术研究不同酵母中HAP 复合体下游调控网络，探索Hap4p调控网络的进化。本研究将对遗传及细胞生物学等领域将产生新的贡献。

中文关键词： HAP复合体；呼吸调控；转录因子调控网络；染色质免疫沉淀-测序；

英文摘要： Under aerobic conditions, eukaryotes mainly assimilate glucose through the respiration pathway in mitochondria for energy metabolism. However, fermentation is the main metabolic pathway for Crabtree-positive yeasts when glucose is present in the environment. The evolution of aerobic fermentation is associated with transcriptional reprogramming. The HAP transcriptional complex is involved in the balance between fermentation and respiration in Saccharomyces cerevisiae. Within the HAP complex, the expression of transcriptional factor Hap4p is induced on non-fermentable carbon sources. Orthologous of the Hap4p from different yeasts only contain two short conserved motifs located in the N and C-terminals, respectively. The rest parts of sequences are quite divergent. In this project, we select the HAP complexes in pre-WGD and Post-WGD yeast species which are responsible for the activation of many genes involved in respiratory metabolism and other metabolic functions as targets. Through studying the expression patterns of the key regulator of the complex activity in different growth conditions, coupling chromatin immunoprecipitation with high-throughput DNA sequencing to identify the regulatory networks of HAP complexes, the goal of this project is to explore the evolution of yeast Hap4p regulatory networks. This work

英文关键词： HAP Complex；Regulation of Fermentation；Transcriptional Networks；Chip-Seq；