奶牛泌乳和乳腺退化的5-HT调控机制研究

项目名称： 奶牛泌乳和乳腺退化的5-HT调控机制研究

项目编号： No.31201115

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 发育生物学与生殖生物学

项目作者： 侯秋玲

作者单位： 山东农业大学

项目金额： 23万元

中文摘要： 5-HT被认为是一种重要的泌乳稳态和退化局部调控因子。但是，对于其调控泌乳及乳腺退化的机制，目前知之甚少。奶牛乳腺表达多种亚型的5-HT受体（HTR1B, 2A, 2B, 4, 和7），选择性阻断HTR1B和HTR2A引起乳蛋白表达量升高。本项目将利用原代培养的奶牛乳腺上皮细胞（Mammary epithelial cells, MEC），探寻HTR1B和HTR2A调控乳蛋白的表达的信号通路；检测5-HT及其各亚型受体选择性拮抗剂对奶牛MEC增殖与凋亡的影响，并探寻其作用机制；确定5-HT是否通过改变IGF-I、 Fas-l、EGFs、TGF-beta?等细胞增殖凋亡调控因子的表达水平间接诱导乳腺退化，以系统揭示5-HT调控奶牛泌乳和乳腺退化的机理。通过探明5-HT的作用机理，有助于进一步揭示乳腺生物学的基本规律，推动泌乳生理学的发展，为提高奶牛乳产量及乳品质提供理论依据。

中文关键词： 血清素（5-HT）；乳腺上皮细胞（MAC-T）；增殖凋亡；信号通路；奶牛

英文摘要： Serotonin (5-HT) has been demonstrated to be an important local regulator of lactational homeostasis and involution. Whereas, the mechanisms employed by 5-HT during regulation of lactation and involution in the bovine mammary gland are poorly understood.The bovine mammary gland expresses multiple functional isoforms of serotonin receptors (HTR1B, 2A, 2B, 4, and 7), and expression of milk protein levels were stimulated by blocking HTR1B and HTR2A with the selective antagonists. In this project, we will use primarily cultured bovine mammary epithelial cells (MEC),to investigate the intracellular signal pathway mediating the serotonin receptors' (HTR1B and HTR2A) action on regulating expression of milk protein; test the effect of 5-HT and selective 5-HT receptor antagonists on proliferation and apoptosis of MEC, and explore which intracellular pathways are involved in the transduction of the serotoninergic intracellular signal; and then determine whether 5-HT induce mammary gland involution indirectly through changing expression level of the factors that regulate proliferation and apoptosis of MEC,such as IGF-I, Fas-l,EGFs and TGF-beta. Addressing the serotonin mechanisms associated with lactation and involution, may help us to unveil general principles of mammary gland biology, promote the development of lactation

英文关键词： Serotonin (5-HT)；mammary epithelial cells (MAC-T)；proliferation and apoptosis；signaling transduction；Bovine