MaPIP1;1介导香蕉响应干旱胁迫的分子调控机制解析

项目名称： MaPIP1;1介导香蕉响应干旱胁迫的分子调控机制解析

项目编号： No.31501371

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 许奕

作者单位： 中国热带农业科学院海口实验站

项目金额： 20万元

中文摘要： 香蕉是受干旱胁迫影响最重的水果之一，干旱胁迫严重制约着香蕉的生产。香蕉对干旱的响应机制研究就成为重要的研究课题。水通道蛋白(aquaporins, AQPs)具有转运水的功能，在植物适应干旱胁迫方面起着重要作用。但是，AQP基因响应干旱胁迫的分子机制尚不清楚。我们前期的研究发现香蕉MaPIP1;1具有抗旱功能。本项目将运用酵母单杂交、凝胶阻滞、共表达分析等生化与分子生物学方法，识别MaPIP1;1启动子区干旱应答元件，鉴定直接调控MaPIP1;1基因表达的转录因子，解析目标转录因子调控MaPIP1;1表达的分子机制，从而揭示MaPIP1;1介导香蕉响应干旱胁迫的分子调控机制。研究结果将拓展AQP基因表达调控机制和香蕉响应干旱胁迫分子机制的理解，并且为香蕉抗旱性遗传改良打下基础。

中文关键词： 草本类；基因克隆；基因表达；遗传机制；抗性育种

英文摘要： Banana is one of the fruits that most affected by the drought stress. Drought stress restricts the banana production seriously. Study on the responsive mechanisms to drought of banana has become the most important research subject. Aquaporins (AQPs) have the function of transporting water, which play an important role in plant to adapt drought stress. To date, it remains unclear for the regulatory mechanism of AQP-mediated drought tolerance. In our previous study, we identified banana MaPIP1;1 can make plant to resistant drought. In this study, we will use yeast one-hybrid, electrophoretic mobility shift assays, co-expression analysis and other biochemical and molecular biology techniques to identify the drought responsive element in MaPIP1;1 promoter region, authenticate the transcription factor that direct regulating MaPIP1;1 expression, uncover the molecular mechanism of key transcription factors regulating MaPIP1;1 expression, the study finally reveal the molecular regulation mechanism of MaPIP1;1 mediated banana in response to drought stress. The findings will expand AQP gene expression regulation mechanism, increase the understanding of banana drought-response molecular mechanisms and provide theoretical basis for genetic improving the resistance of banana drought.

英文关键词： Herb;Gene cloning;Gene expression;Genetic mechanisms;Resistance breeding