矮牵牛RCW基因调控花瓣呈色的分子机理研究

项目名称： 矮牵牛RCW基因调控花瓣呈色的分子机理研究

项目编号： No.U1504320

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 张和臣

作者单位： 河南省农业科学院

项目金额： 27万元

中文摘要： 花瓣的颜色是植物的重要观赏性状。研究表明，多数植物的花瓣呈色是花青素在花瓣细胞中积累的结果。在矮牵牛中发现，编码WRKY、MYB、bHLH、WD40的转录因子可以相互作用，形成复合体WMHW，调控花青素的合成。我们同时发现该复合体可以调控RCW基因的转录。在矮牵牛M1×V30中通过RNAi沉默该基因的表达，可以使花瓣呈现更深的紫色。因此,我们推测RCW基因受WMHW的转录调控，参与矮牵牛的花瓣呈色过程。为了进一步验证该论断，我们申请该项目。本研究以RNAi、基因超表达为手段，通过分析转基因后代的表型特征，解析RCW基因对花瓣呈色的作用；并通过酵母双杂交、BiFC、激光共聚焦显微成像等技术分析其与花瓣呈色相关基因之间的关系，鉴定RCW在细胞中作用的位置，从而阐明RCW基因的分子功能。研究结果不仅可作为花瓣呈色分子调控理论体系的有益补充，也可为将来通过分子育种手段改变花色提供重要的理论依据。

中文关键词： 矮牵牛；色素；基因功能；基因调控；花青素

英文摘要： The coloration of petal is one of important ornamental characters of plants. It has been identified that most flower colors were primarily due to anthocyanin that was accumulated in the petal cells. The transcription factor complexes of WRKY, MYB, bHLH and WD40 (WMHW) can interact with each other and regulate the synthesis of anthocyanin. In previous works, we also found the gene of RCW can be regulated by the regulator complexes in Petunia. To silence RCW gene in Petunia × hybrid M1×V30 through a RNAi process can produce more deeper violet petal. We deduced that this gene, regulated by the WMHW complexes, may be involved in the petal pigmentation process. For further verifying the assertion, we need more experiments, and therefore apply for this project. In this research, we will identify the gene function related to the pigmentation by analyzing the phenotype of the RCW- RNAi or over-expression plants. In addition, we will also carry on the gene functional analyses by for instance using the yeast-2 hybrid and BiFC to investigate the interaction of RCW and other pigmentation related proteins, and using the confocal fluorescence technique to detect RCW sub-cellular localization. The proposed project will not only deliver new components of the mechanism underlying regulation of the petal pigmentation, but also give an important foundation for further developing genetic engineering of flower color of ornamental plants.

英文关键词： Petunia;Pigment;Gene function;Gene regulation;Anthocyanin