过氧化物酶体增殖物激活受体α对青光眼视网膜神经节细胞的保护作用及机制的研究

项目名称： 过氧化物酶体增殖物激活受体α对青光眼视网膜神经节细胞的保护作用及机制的研究

项目编号： No.81500720

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 丁乐溪

作者单位： 中南大学

项目金额： 18万元

中文摘要： 视网膜神经节细胞(RGCs) 的选择性、进行性死亡是青光眼视神经损伤的共同通路和最终结局。近年来研究发现视乳头微循环障碍会直接导致视乳头缺血缺氧，从而引起RGCs氧化应激，诱导RGCs凋亡。有研究证实，过氧化物酶体增殖物激活受体α（PPARα）及其激动剂通过抑制氧化应激等机制对中枢神经性疾病具有一定的治疗作用，但其对于青光眼的视神经保护作用鲜有报道。本课题组率先发现PPARα通过抗氧化应激机制对于糖尿病视网膜血管病变和视神经病变具有显著保护作用，并且发现PPARα在青光眼中表达异常，因此提出假设PPARα可通过抗氧化应激机制抑制青光眼诱导的RGCs凋亡，从而对青光眼视神经损害具有保护作用。本项目拟通过细胞和青光眼动物模型，首次明确PPARα及其激动剂对RGCs凋亡的调控作用及机制，推动青光眼视神经保护研究的发展，为青光眼视神经保护提供新的治疗靶点和用药思路，为青光眼治疗带来新的希望。

中文关键词： 青光眼；视网膜神经节细胞；凋亡；氧化应激；线粒体

英文摘要： The common pathway and the final outcome of glaucoma is selective and progressive death of retinal ganglion cells (RGCs). Growing evidence suggests that microcirculation disorder will induce hypoxia directly in optic papilla which can lead oxidative stress resulting in RGCs apoptosis. Peroxisome Proliferator-Activated Receptor α(PPARα) has been comfirmd as a key role in regulating inflammatory and oxidant activities. Recently, many studies demonstrated the beneficial effect of PPARα agonist on central nervous system disease through an anti-oxidant mechanism. However, the effect of PPARα on optic nerve in glaucoma was barely reported. We has firstly demonstrated the protective effect of PPARα on diabetic retinopathy and neuropathy by exerting anti-oxidant capability. We also found the change of PPARα expression in glaucomatous animal model. Therefore, we hypothesize PPARα plays a benificial role in glaucoma-induced RGCs apoptosis and optic damage in an anti-oxidant mechanism. In this study, we will firstly investigate the effect and the uderlying mechanism of PPARα as well as its agonist on glaucoma-induced RGCs apoptosis using glaucomatous cell and animal model, in order to develop the neuroprotection of glaucoma, provide new therapeutic gene target and drug method, enlight the future of glaucoma treatment.

英文关键词： glaucoma;retinal ganglion cells;apoptosis;oxidative stress;mitochondria