项目名称: 多肽末端保护微阵列分析及肿瘤标志物高通量传感方法研究
项目编号: No.21505040
项目类型: 青年科学基金项目
立项/批准年度: 2016
项目学科: 数理科学和化学
项目作者: 文茜
作者单位: 湖南大学
项目金额: 21万元
中文摘要: 肿瘤标志物的分析检测对恶性肿瘤的早期诊断和预后跟踪意义重大。发展高通量、高灵敏、低成本检测技术是肿瘤标志物分析领域的挑战性任务。本项目拟将生物传感技术、多肽微阵列芯片技术以及纳米探针技术相结合,通过构建多种不同类型肿瘤标志物与多肽的识别模型,深入探索研究蛋白与多肽特异识别结合后对肽酶活性影响,阐明多肽末端保护效应及机理;并以特异性多肽为识别元件,结合金属纳米材料优异的信号转换和信号放大性能,构建一种高灵敏、无标记的新型生物传感体系用于肿瘤标志物检测。同时,结合多肽微阵列芯片技术,发展高通量、高特异性、高灵敏的多种不同类型肿瘤标志物的快速分型筛选及定量检测分析方法;并将该多肽末端保护微阵列生物传感分析方法应用于临床样品检测中,实现多种不同类型肿瘤标志物的高通量、多指标、快速检测。总体而言,本项目研究不仅具有较高的学术价值,也具有重要的社会意义和广阔的临床应用前景。
中文关键词: 多肽末端保护;微阵列芯片;肿瘤标志物;纳米探针;高通量分析
英文摘要: The detection and characterization of tumor biology markers holds enormous promise for early diagnosis and optimizing systemic therapy. It has been a great challenge to develop sensitive and high-throughput assay methods for tumor markers detection in chemistry and biology. Via combining the advantages of biosensor technology, peptide microarray biochip technology and nanoprobe technology, the project focuses on the development of novel detection tumor marker assay platform. In this study, we will try to illuminate the mechanism of terminal protection of peptide by establishing different model of tumor biology marker and peptide. Establishment of a novel biosensor system with high sensitive and label free by using specificity peptide as recognition module and nano-metallic materials be the signal converter and signal amplifier. Based on this nanoprobes biometric system, combined to the peptide microarray technique, develop a high throughout、high specificity method for multiplex detection of tumor marker in clinical samples. These principles form the basis for rapid, robust, low-cost and high-throughput tumor markers assay platform. Briefly, significant theoretical and practical values are expected.
英文关键词: terminal protection of peptide;microarray biochip;tumor markers ; nanoprobe ;high-throughput assay