益气养阴消癥通络法对糖尿病肾病大鼠及高糖诱导足细胞的影响

项目名称： 益气养阴消癥通络法对糖尿病肾病大鼠及高糖诱导足细胞的影响

项目编号： No.81202829

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 王月华

作者单位： 河北医科大学

项目金额： 23万元

中文摘要： 肾为络脉聚集之所，"久病入络"为肾脏病发展的中心环节之一。我们以"久病入络"理论为指导，提出气阴两虚、癥积络阻为糖尿病肾病（DN）基本病机，以益气养阴消癥通络为DN的治疗大法。而前期研究中我们已经证实该法能够通过对DN大鼠TGF-β/Smads、氧化应激及RAS系统的影响发挥对DN的治疗作用。现代研究表明，足细胞及其裂孔隔膜上的裂孔膜蛋白与蛋白尿的发生密切相关，而血管内皮生长因子(VEGF)是迄今所发现最强大的血管通透因子，能增加肾小球滤过屏障对血浆蛋白的通透性，其过表达参与了DN蛋白尿的发生。因此本研究继以DN动物模型、高糖培养足细胞为研究对象，从分子水平、组织水平和动物体内实验，观察益气养阴消癥通络法对DN足细胞及裂孔膜蛋白以及对VEGF信号转导通路的影响，为中药复方制剂延缓DN提供现代医学理论依据，而且通过该法防治DN的机理研究，将以现代细胞、分子生物学理论阐释传统中医的辨证论治。

中文关键词： 糖尿病肾病；益气养阴消癥通络中药；足细胞；裂孔膜蛋白；血管内皮生长因子信号通路

英文摘要： Kidney is to assemble collaterals. "Pathogen usually intruding into collateral in protracted disease" is a center link of the development in renal disease.Under the theory of "Pathogen usually intruding into collateral in protracted disease", We proposed deficiency of both vital energy and yin and the collateral blood stasis for the basi-pathogenesis of diabetic nephropathy.We also proposed Qi Supplementing, Yin Nourishing, Blood Stasis Dispersing, Collateral Dredging (QYBC) for the therapeutic principle of diabetic nephropathy. We had known that QYBC had therapeutic action on DN by TGF-β/Smads, oxidative stress and RAS. The modern research indicated that podocyte and its slit membrane protein had the close correlation to proteinuric development. VEGF is the best powerful blood vessel permeation factor which raise the permeability of glomerular filtration for glomerular filtration and participate in the proteinuric development of DN. Therefore, this research view QYBC for podocyte and its slit membrane protein and VEGF signal transduction iteral influence by animal experiment, which provide theory evidence of modern medicine for traditional Chinese medicine compound preparation. Furthermore, to elucidate determination of treatment based in pathogenesis obtained throu-gh differentiation of symptoms and signs of

英文关键词： Diabetic nephropathy；The traditional Chinese medicine of yqyyxztl；Podocyte；Slit diaphragms ptotein；VEGF signaling pathway