海带多糖通过调节vWF表达，保护放射性脑损伤的研究

项目名称： 海带多糖通过调节vWF表达，保护放射性脑损伤的研究

项目编号： No.81460479

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 韦力

作者单位： 广西医科大学

项目金额： 45万元

中文摘要： 放射性脑损伤是头颈部肿瘤放射治疗过程中最常见的并发症，长期以来严重地困扰着临床医生。我们的前期工作表明，放射致内皮细胞损伤是脑继发损伤的主要原因，但其机制尚未清楚。我们进一步发现海带多糖可显著改善放射性脑损伤的记忆功能障碍。在肾上腺素致内皮损伤大鼠模型中，海带多糖可通过下调内皮细胞分泌的血管性血友病因子（vWF）拮抗内皮损伤大鼠血栓形成。因此，海带多糖也很可能通过影响vWF的表达对放射性脑损伤起保护。为证实这一观点，本项目拟建立放射性脑损伤的大鼠模型，采用神经行为学、细胞和分子生物学等技术手段，从整体和细胞水平，观察vWF表达、血栓形成、神经细胞变化和干预效果，分析vWF与血栓形成和神经细胞病理变化的关系, 探讨海带多糖对放射性脑损伤保护的分子机制，为海带多糖运用于临床防治放射性脑损伤提供理论依据。

中文关键词： 放射性脑损伤；海带多糖；内皮细胞；血管性血友病因子(vWF)；血栓形成

英文摘要： Radiation brain injury is a common and serious clinical complication after radiation therapy for brain and neck tumor, and seriously troubles clinicians for a long time. Our previous study indicated that secondary brain injury was mainly caused by injured endothelial cells after radiation, but its mechanism remain unclear. In addition, our research further revealed that laminaria polysaccharide significantly improved memory disorders of mouse following radiation. In rats model with endothelial cells injuried by adrenaline, laminaria polysaccharide was known to reduce the expression of von Willebrand factor (vWF) secreted by endothelial cells, and played a role in ant thrombosis. Therefore, we hypothesize that laminaria polysaccharide maybe regulate the expression of vWF , and offer neuroprotection for radiation-induced brain injury. To confirm this view, rat's model with radiation-induced brain injury will be established in vitro and in vivo. Following laminaria polysaccharide, neurological behavior, cell and molecular biology technique, and so on, will be employed to observe the expression of vWF, thrombosis , and the changes of nerve cell and cerebral function. we will come up with the molecular mechanism of laminaria japonica polysaccharides in protection of radiation brain injury by analyzing the relationship among vWF , thrombosis and nerve cell . After this research, a theoretical basis of laminaria polysaccharide in prevention and treatment of radiation brain injury will be provided for physicians.

英文关键词： radiation brain injury;Laminaria Polysaccharide;endothelial cells;vow willwbrand factor(vWF);thrombosis