基于宏基因组学的黄土高原退耕还林区土壤微生物群落的时空响应

项目名称： 基于宏基因组学的黄土高原退耕还林区土壤微生物群落的时空响应

项目编号： No.31470534

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生态学

项目作者： 林雁冰

作者单位： 西北农林科技大学

项目金额： 86万元

中文摘要： 本研究针对土壤微生物多样性在黄土高原退耕还林过程中时空响应这一科学问题，采用宏基因组学（高通量测序技术）和传统方法相结合的方式，拟针对退耕还林过程中不同年限（0-82年）、不同坡面植被演替阶段的土壤细菌和真菌多样性时空分布进行研究，力图从多个层面利用系统学分析获得的土壤细菌和真菌的物种遗传、结构、功能多样性及分子生态学信息，揭示土壤微生物群落多样性与退耕还林后植被和土壤之间的相互关系，阐明土壤微生物多样性对黄土高原不同环境条件变化的响应机制, 以期为更好地维持土壤生态系统的稳定性及其生态服务功能提供理论依据。

中文关键词： 高通量测序；土壤微生物多样性；宏基因组学；退耕还林；植被恢复

英文摘要： This study will focus on the temporal and spacial of the soil microbial diversity during the process of the conversion of cropland to forest project on the loess plateau. By using metagenomics (high-throughput sequencing technologies) and the traditional methods, the temporal（0-82a） and spacial distribution of the soil bacteria and fungi diversity were studied during the conversion of cropland to forest in different ages, different stages of vegetation succession. The genetic, structure and function diversity of soil bacteria and fungi and molecular ecology information were obtained through the analysis of systematic from the different levels. The results will reveal the relationship between the soil microbial community diversity and vegetation, soil after the conversion of cropland to forest. Proclaim the response mechanism of soil microbial diversity to the different environmental conditions change on the loess plateau. It will also provide the scientific proofs for better maintain the stability of soil ecosystem and its ecological service function.

英文关键词： High-throughput sequencing;soil microbial diversity;metagenomics;conversion of cropland to forest;revegetation