共生对爪哇伪枝藻抵御荒漠胁迫能力的影响机理

项目名称： 共生对爪哇伪枝藻抵御荒漠胁迫能力的影响机理

项目编号： No.31300100

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 吴丽

作者单位： 武汉理工大学

项目金额： 23万元

中文摘要： 生物结皮具有防治土壤侵蚀、沃土增肥等重要功能，并且这些功能会随着结皮发育演替逐步增强。共生关系是推动结皮发育演替的一个重要因素。爪哇伪枝藻不仅是生物结皮演替早期的先锋种类之一，它也可以同真菌共生形成地衣而在演替后期占据优势地位。本项目拟以共生态、非共生态爪哇伪枝藻为研究对象，通过模拟野外荒漠胁迫条件，利用活体叶绿素荧光技术、低温荧光检测技术以及HPLC等技术，对比研究荒漠胁迫条件对共生态、非共生态爪哇伪枝藻光合系统结构及功能活性、抗氧化系统效率及次生代谢产物积累的影响，从而揭示二者在抗干旱、抗高光强以及抗UV胁迫能力方面的差别，并探究其内在机制。研究结果不仅能够揭示共生关系对藻类抗荒漠胁迫能力的影响机理，从结皮藻类环境适应性角度揭示生物结皮发育演替机理，也将为生物结皮在荒漠化治理中的应用提供重要理论基础。

中文关键词： 生物土壤结皮；共生；爪哇伪枝藻；环境适应性；

英文摘要： Biological soil crusts (BSCs) have important ecological functions, such as avoiding soil erosion and fertilizing soil, and these functions will be gradually improved with succession. Symbiosis is one of the important factors that accelerate BSCs succession. Free-living filamentous Scytonema javanitum not only colonizes the early stage as the pioneer species, but also dominates the later stage as the photoboint of lichens. Two different living forms, free-living and symbiotic S. javanicum, will be studied in this project. Under the indoor simulated conditions, the effects of desert stresses on photosynthetic system structures and their activities, the efficiency of antioxidant system and the accumulation of secondary metabolites will be well studied, using multiple technologies, such as in vivo chlorophyll fluorescence, 77K low temperature detection and HPLC technology, aiming at comparing the abilities in resisting desiccation, high visible radiance and UV radiance between free-living and symbiotic S. javanicum. These results will not only reveal the effect mechanism of symbiosis on the adaption ability of S. javanicum to desert stresses, uncoving the successional mechanism of BSCs from the perspective of cyanobacterial environment adaption, but also provide important theory foundation for the usage of BSCs in

英文关键词： Biological soil crusts；Symbiosis；Scytonema javanicum；environmental adapbility；