猴脑选择性深低温断血流复苏对认知功能影响的研究

项目名称： 猴脑选择性深低温断血流复苏对认知功能影响的研究

项目编号： No.81460174

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 蒲军

作者单位： 昆明医科大学

项目金额： 47万元

中文摘要： 临床使用的深低温停循环技术，其断血流的安全时限常不能满足临床工作的需要，且易引起心肺并发症和认知功能损害。研究表明：阻断猴双侧颈总动脉后行顺行性脑灌注，降低脑温至18℃，体温保持在33℃以上，使脑选择性降温，可延长脑对血流阻断的安全时限，对全身主要器官无明显损害，但对认知功能的影响缺乏了解。本研究拟在改进脑选择性深低温断血流复苏模型的基础上，采用事件相关电位和脑电对脑选择性深低温断血流复苏前后与认知相关的神经电生理活动进行研究；用MRI动态观察有无缺血梗塞灶；用1H-MRS检测复苏前后与认知相关的前额叶及海马的神经元密度和活性变化；并采用延缓反应来进行猴工作记忆的检测。其结果有助于从认知功能角度认识选择性深低温脑保护技术的有效性和安全性，为临床应用此项技术提供实验基础和理论依据。

中文关键词： 脑缺血；选择性深低温；猕猴；事件相关电位；认知

英文摘要： At present, whole body deep hypothermic circulatory arrest(DHCA) was used clinically. However, there are many complications that limit its broad use, e.g. the interval of blood flow occlusion is usually less than 60 min. Studies have shown that: antegrade cerebral perfusion after total blockage of macaca mulatta's bilateral carotid artery, reduced brain temperature to 18 ℃, the temperature maintained above 33 ℃, the brain is selectively cooled and the brain may extend the time limit for blood safety blockage, the main organs of the body will not be significantly damaged, but the impact on cognition is not clearly understood. However, cerebral selective deep hypothermia combined with antegrade cerebral perfusion can not only avoid these side effects effectively but also extend the limitation of blood flow occlusion by more depressed metabolism in macaca mulatta whicn is not sure if there is cognitive dysfunction.We established the model of macaca mulatta of resuscitation after selective cerebral deep hypothermia and blood flow occlusion in macaca mulatta , on the base of this, We improve the ways of preparing the model of macaca mulatta. In order to explore the mechanism which reduce the cognitive dysfunction and is resistant to hypoxia-ischemia, we do something as follows:1.To study event-related potentials(ERP) and electroencephalogram(EEG) of macaca mulatta of resuscitation after selective cerebral deep hypothermia blood flow occlusion 2. To investigate magnetic resonance imagine(MRI) and magnetic resonance spectroscopy(1H-MRS)related to cognition of macaca mulatta of resuscitation after cerebral selective deep hypothermic and blood flow occlusion, 3.To Use delayed response experiment detects working memorys. All of these we do is to explore the mechanism of selective cerebral deep hypothermia reducing the complications and being resistant to hypoxia-ischemia in different aspects, which let us to use it rationally in clinical in the future.

英文关键词： cerebral ischemia;selective deep hypothermia;macaca mulatta;event-related potential;cognition