IL-17A在肺炎链球菌急性中耳炎黏膜免疫保护中的作用

项目名称： IL-17A在肺炎链球菌急性中耳炎黏膜免疫保护中的作用

项目编号： No.81200736

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学二处

项目作者： 徐江红

作者单位： 复旦大学

项目金额： 23万元

中文摘要： 肺炎球菌是急性中耳炎最常见病原菌，疫苗是减少中耳炎的有效手段，抗体一直被认为是肺炎球菌疾病的主要防御机制，而目前市场上现有的主要产生抗体的疫苗对急性中耳炎的保护力很有限。IL-17A是调节固有免疫和获得性免疫的重要因子，已有研究证实IL-17A在预防肺炎球菌鼻咽部定植中的作用，但在急性中耳炎黏膜免疫中的作用无相关研究。前期我们采用新型肺炎球菌蛋白疫苗经鼻腔途径免疫小鼠，已证实对急性中耳炎的保护作用，并初步发现黏膜中IL-17A的分泌增多，本课题将探讨IL-17A在肺炎链球菌急性中耳炎黏膜免疫保护中的作用，研究内容为1）建立肺炎球菌急性中耳炎动物模型，研究IL-17A在急性中耳炎固有免疫中的作用；2）新型肺炎球菌蛋白疫苗黏膜途径免疫，研究IL-17A在适应性黏膜免疫保护的作用。通过上述研究，力图阐明IL-17A是肺炎球菌急性中耳炎黏膜免疫的主要防御机制，为新一代疫苗的设计及免疫策略提供依据。

中文关键词： IL-17A；中耳炎；黏膜免疫；肺炎链球菌；疫苗

英文摘要： Streptococcus pneumoniae is the most common cause of acute otitis media. Vaccine may be an effective strategy in the prevention of pneumococcal diseases. Antibody has been long considered to play a key role on prevention of pneumococcal diseases. However, the pneumococcal vaccines which mainly induce antibody in the market play limited protective roles on acute otitis media. In recent years, studies have confirmed IL-17A mediated acquired immunity to pneumococcal colonization. However, the role of IL-17A in mucosal immunity against pneumococcal otitis media remained unknown.BALB/c mice were immunized intranasally with pneumococcal surface antigen A (PsaA) using chitosan as an mucosal delivery system and the protecive roles against pneumococcal otitis media have been confirmed in our previous studies. We also found that the IL-17A was higher in the bronchoalveolar lavage fluids in the vaccined groups than in the non-vaccined groups in our preliminary studies. Our future studies will focus on the evaluation of roles of IL-17A in mucosal immunity against pneumococcal otitis media.The research contents as follows:1) The establishment of animal models of acute pneumococcal otitis media and the roles in innate immunity of pneumococcal otitis media; 2) Mice were immunized intranasally with PsaA vaccines and the roles

英文关键词： Interleukin-17A；Otitis media；Mucosal immunity；Streptococcus pneumonia；Vaccine