植物单宁影响布氏田鼠取食行为的神经机制

项目名称： 植物单宁影响布氏田鼠取食行为的神经机制

项目编号： No.31470017

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 杨生妹

作者单位： 扬州大学

项目金额： 30万元

中文摘要： 植物次生化合物在动植物的协同进化中发挥着重要作用。单宁是重要的次生化合物，影响动物的取食行为。但单宁影响动物取食行为的神经机制尚无研究。本项目将在口腔给予不同剂量单宁酸后，观察的布氏田鼠的取食行为，确定单宁对布氏田鼠取食行为的影响；测定其血液中皮质酮含量、中脑腹侧被盖区及伏膈核等部位各类神经递质含量和多巴胺结合受体数目，确定单宁对动物奖赏系统的影响；测定血液中瘦素水平和下丘脑MCH、Orexin、POMC、AGRP及NPY等神经肽的表达，确定单宁对动物摄食中枢的作用。通过奖赏系统及摄食中枢功能与取食行为间的相关性分析，揭示单宁影响动物取食行为的神经机制，为动植物间的协同进化提供理论依据。

中文关键词： 协同进化；神经机制；取食行为；单宁；布氏田鼠

英文摘要： The plant secondary metabolites (PSM) play an important role in the coevolution of the plants and animals. Tannins are important PSM and they influence the feeding behavior of animals. But there is few about the study of the neural mechanism of the effect of tannins on the feeding behavior. The subjects of this project were there kinds of animals, they were Brandt's voles (Lasiopodomys brandtii) which could select different plants in the filed, Brandt's voles fed on unique plant with different content of tannins or Brandt's voles given different doses of tannin acid through oral, respectively. At first, the feeding behavior of these animals would be observed to confirm the effect of tannins on the feeding behavior. Secondly, in order to study the perception and transduction path of the gustative system to tannins, the change of the anatomic structure of tongue taste buds, the number of gustation cell, the gustation receptors and the signal transduction substance of bitter would be compared among there kinds of animals just mentioned. Thirdly, to explore the effect of tannins on the rewards system, the level of plasmic corticosterone, the content of some neural transmitters and the number of dopamine receptor in ventral tegmental area and nucleus accumbens would be assayed among three kinds of animals. Fourthly,

英文关键词： coevolution；neural mechanism；feeding behavior；tannins；Brandt's vole