下丘脑室旁核内NMDA和GABA受体介导中枢交感神经兴奋对射精行为影响的研究

项目名称： 下丘脑室旁核内NMDA和GABA受体介导中枢交感神经兴奋对射精行为影响的研究

项目编号： No.81501245

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 夏佳东

作者单位： 南京医科大学

项目金额： 18万元

中文摘要： 原发性早泄和延迟/不射精一直是困扰着男性性功能健康的常见疾病，其具体发病机制并不清楚。近年来，临床研究发现中枢交感神经高兴奋性可能与原发性早泄的发生相关，应用降低交感神经药物（如SSRI类药物）可延长阴道内射精潜伏期。我们设想中枢交感神经兴奋状态可能影响着性生活时的射精潜伏期。而下丘脑室旁核（PVN）内的NMDA和GABA是参与调节中枢交感神经兴奋性两种主要受体。本课题拟通过干预PVN核团内的NMDA和GABA受体以调节中枢交感神经兴奋状态，并应用中枢靶向干预、机能学和行为学技术，探讨中枢交感神经兴奋状态与射精潜伏期之间的关系；此外，探讨不同射精潜伏期雄鼠之间的中枢交感神经兴奋状态特点及其差异情况，并通过神经分子生物学技术进一步讨论可能的机制。通过上述方法，系统研究中枢交感神经高兴奋在射精发生中的作用及其机制。实验结果将从新的视角阐释原发性早泄和延迟/不射精性功能障碍复杂的病理生理机制。

中文关键词： 射精功能障碍；下丘脑室旁核；；中枢交感神经；性行为

英文摘要： Primary premature ejaculation (PE) and delayed ejaculation/ anejaculation (DE/AE) are the most common male sexual dysfunctions, yet their specific mechanisms are unclear. Recent studies have suggested that primary PE could be caused by the hyperactivity of central sympathetic nervous system (CSNS), and intravaginal ejaculation latency time could be prolonged through reducing CSNS activity (such as SSRIs). We assume that the states of CSNS could be associated with ejaculation latencies, and NMDA and GABA receptor in hypothalamic paraventricular nucleus (PVN) are the main receptors, which mediate the states of CSNS. In this study, we will regulate the states of CSNS through NMDA and GABA receptors in PVN and observe their effects on ejaculation behavior, using a Central Nervous System Targeting technology, ergology and ethology; in addition, we will also investigate the differences in variable ejaculatory behavior male rats and the possible mechanisms with the molecular biology method. Through the above methods, we systematically studied the effects and mechanisms of CSNS activities on ejaculation. This study is designed to provide new insights in the complex pathophysiological mechanisms of the primary PE and DE/AE.

英文关键词： ejaculatory dysfunction;hypothalamic paraventricular nucleus;central sympathetic nervous;sexual behavior