食品中真菌毒素的多元、高通量纸基免疫层析微流控芯片检测方法研究

项目名称： 食品中真菌毒素的多元、高通量纸基免疫层析微流控芯片检测方法研究

项目编号： No.31201357

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品化学残留与控制

项目作者： 张道宏

作者单位： 西北农林科技大学

项目金额： 23万元

中文摘要： 微流控芯片是当前微全分析系统发展的热点领域，目前已发展为世界上最先进的技术之一。但传统微流控芯片的制作成本较高，限制了其大规模应用。近几年发展起来的纸基微流控芯片以滤纸为基材，更有利于在资源匮乏条件下使用，已被应用于化学、生物、医学等领域，然而，在食品安全领域的应用却鲜见报道，而且其主要采用的双抗体夹心分析模式也很难应用于引起食品安全问题的小分子化合物的检测。针对这一现状，本项目将免疫层析试纸与纸基微流控芯片相结合，研制纸基免疫层析微流控芯片。该芯片采用竞争分析模式，兼具有传统微流控器件通量高的优点，又结合了免疫层析试纸适用于小分子化合物检测的特点，能够实现食品中多种分析物的快速、高通量检测。本项目以真菌毒素为目标分析物，研究内容主要包括三种真菌毒素（黄曲霉毒素B1、赭曲霉毒素A、玉米赤霉烯酮）抗原、抗体的研制；纳米金及其标记物的研制；纸基免疫层析微流控芯片的研制及在食品中的应用。

中文关键词： 赭曲霉毒素；单克隆抗体；快速检测；纸基微流控芯片；

英文摘要： Microfluidic chip is one of the hot fields of "Micro Total Analysis system (μ-TAS)", and has been developed to be one of the most advanced technologies in the world. But the high fabrication cost of traditional microfluidic chip limited its scale application. The paper-based microfluidic chip developed in recently years, using filter paper as the microfluidic substrate is more suitable to be applied in low resource settings and has been used in the fields of chemistry, biology and medicine. However, the application of paper-based microfluidic chip in the field of food safety is barely reported, and the double sandwich assay format mainly adopted by conventional paper-based microfluidic devices is difficult being used in the detection of small molecular compounds which often cause problems of food safety. According to the present situation, we will combine the immunochromatographic strip with paper-based microfluidic chip to develop a paper-based immunochromatographic microfluidic chip. The novel chip using competitive assay format will not only have the advantages of high-throughout of conventional microfluidic chip but also possess the properties of paper test strips being suitable for small molecular compounds detection, and can realize quick and high-throughout detection of multiplex small molecular compounds

英文关键词： Ochratoxin；Monoclonal antibody；Rapid detection；Paper-based microfluidic analytical device；