肾虚血瘀型膝OA力学微环境稳态的软骨阻尼特性研究

项目名称： 肾虚血瘀型膝OA力学微环境稳态的软骨阻尼特性研究

项目编号： No.81673994

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 医药、卫生

项目作者： 刘爱峰

作者单位： 天津中医药大学

项目金额： 25万元

中文摘要： 膝骨关节炎（OA）以中老年为主，肾虚血瘀型多见。前期研究构建了肾虚血瘀型膝OA集骨、软骨、韧带和肌肉的肌骨模型，发现了膝部筋骨应力分布非线性特征，软骨修复需要力学微环境稳态。本项目通过筋骨应力非线性特征，采用生物力学测试、逆向工程技术构建仿真实体模型。对实体模型行滑动坡度、接触面积、润滑液干预实验，得出膝OA力学微环境稳态的最佳干预数据和软骨阻尼参数。采用膝OA模型、肾虚模型、血瘀模型，复合成肾虚血瘀型动物模型。把换算后干预数据加载于动物模型，运用生化、病理、基因表达、步态分析评价软骨修复程度和验证软骨阻尼。结合生物耦合理论，对力学微环境稳态软骨阻尼特性进行论证，证明力学微环境稳态软骨阻尼非线性特点，阐明力学微环境稳态软骨阻尼动态平衡特征，揭示力学微环境稳态的软骨阻尼特性机制。为研究筋骨并重理论提供客观实体模型，为临床诊治肾虚血瘀型膝OA贡献定量诊断证据和定性治疗思路。

中文关键词： 肾虚血瘀；膝骨性关节炎；力学微环境；关节软骨；阻尼特性

英文摘要： The knee osteoarthritis is merely among middle aged and elderly people whose symptoms are usually renal deficiency and blood stasis. Based on the preliminary study, renal deficiency and blood stasis type knee has been constructed, osteoarthritis musculoskeletal model, combining bone, cartilage, ligament and muscle as well. The form study also discovered the stress distribution’s nonlinear characteristic of ligament and bone in knee, and cartilage repair needs stable dynamical microenvironment. Based on the nonlinear characteristic mentioned above, this project will construct solid simulation model with the adoption of biomechanical test and reverse engineering. By testing the model with sliding slope, contact area and lubricant experiment, the study would get the optimum intervention data and cartilage damping parameter of knee osteoarthritis’s microenvironment. With the help of compounded knee osteoarthritis model, renal deficiency model, blood stasis model to the renal deficiency and blood stasis animal model, the study would loaded revaluated data to animal model. Cartilage repairing condition would be analyzed and cartilage damping would be verified according to biochemical index, pathological section,gene expression and gait analysis as assessment methods. On the basis of biotic coupling theory, we will demonstrate the cartilage damping characteristic in dynamical microenvironment. In this way, the study proves the nonlinear characteristic of cartilage damping in dynamical microenvironment, elucidates homeostasis characteristic of cartilage damping in dynamical microenvironment and finally reveals the mechanism of cartilage damping in dynamical microenvironment. This project will provide objective solid model for the emphasis on both sinews and bones, and give quantitative diagnosis evidences as well as qualitative treatment approaches for the diagnosis and treatment of renal deficiency and blood stasis type knee osteoarthritis.

英文关键词： Kidney and Blood stasis;Knee Osteoarthritis;Mechanical Micro environment; Cartilage;Damping Characteristics