西瓜低温诱导转录因子ClMYB的功能鉴定及其调控机制解析

项目名称： 西瓜低温诱导转录因子ClMYB的功能鉴定及其调控机制解析

项目编号： No.31471894

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 孔秋生

作者单位： 华中农业大学

项目金额： 80万元

中文摘要： 低温是西瓜早春生产中的主要非生物限制因子，MYB转录因子广泛地参与了植物对逆境胁迫的响应。在前期的研究中，我们在西瓜全基因组上鉴定出154个MYB家族转录因子，分析了其中88个R2R3类MYB转录因子对低温胁迫的响应，发现一个转录因子（暂称为ClMYB）受诱导上调表达，并在耐低温和低温敏感材料之间存在着显著的差异，推测该转录因子与西瓜耐低温性状有关，我们克隆了该转录因子。在此基础上，本项目拟首先分析ClMYB转录因子的基本特征；分别转化烟草和野生西瓜验证其耐低温功能；然后利用RNA-seq、凝胶阻滞分析和酵母单杂等手段，发掘ClMYB调控的靶基因，分析其作用的分子和生理机制。本项目是已有研究的深入和延续，研究结果将为西瓜耐低温种质创新提供重要的基因资源，同时也为解析以ClMYB转录因子为节点的低温胁迫应答网络提供理论依据。

中文关键词： 西瓜；转录因子；低温胁迫；ClMYB；调控机制

英文摘要： Low temperature is one of the major limiting factors in watermelon production in early Spring. Transcription factors in MYB family extensively participate in the responses to abiotic stresses in plants. In our previous work, 154 transcription factors in MYB family were identified on watermelon genome. Of them, 88 MYB genes belong to the R2R3 type and their responses to low temperature stress were analyzed in watermelon. Expression level of a MYB gene (which was temporarily named as ClMYB) was found to be upregulated under low temperature stress. Significant expression levels were also observed for ClMYB between the tolerant and susceptible genotypes under low temperature stress. Those results suggested the potential function of low temperature for ClMYB gene. Based on these data, in the present project we will firstly identify the fundamental characteristics of ClMYB as a transcription factor. Then, transformation of tobacco and wild watermelon will be conducted to validate the function of low temperature tolerance for ClMYB. Meanwhile, RNA-seq, EMSA, and yeast hybrid system will be used to indentify the target genes of ClMYB. At last, physiological measurement with regard to some metabolic pathways involving the differentially expressed genes will be carried out. The current project is an extension of our earlier work. It will lay theoretical groundwork for deciphering the mechanisms of action on ClMYB, tapping its target genes, and setting up a low temperature stress-responsive network using ClMYB as a node. Furthermore, it will provide valuable gene resource for innovation of watermelon germplasm with low temperature tolerance.

英文关键词： watermelon;transcription factor;low temperature stress;ClMYB;regulatory mechanism