基于掺杂型ZnS/Au纳米复合材料的免疫检测研究

项目名称： 基于掺杂型ZnS/Au纳米复合材料的免疫检测研究

项目编号： No.61205193

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 信息四处

项目作者： 楚学影

作者单位： 长春理工大学

项目金额： 28万元

中文摘要： 半导体纳米晶由于具有发光波长可调、发射谱窄、较好的光稳定性、激发谱连续等优异的光学性质，近些年来在生物分子及细胞等的标记检测领域中引起了人们极大的重视。环境安全问题和材料体系单一是当前制约半导体荧光标记与检测技术的主要因素。针对这一问题，本项目拟开展可应用于生物分子检测的新型荧光纳米复合材料的研究工作。主要包括水相方法制备水溶性掺杂型ZnS纳米材料，通过掺杂元素及掺杂浓度调节实现对其光学性质调控。以此为基础利用多齿配体的桥梁作用构建掺杂型ZnS/Au纳米复合材料。利用Au纳米材料易与生物分子偶联及可在光激发下产生等离子体场的特性，实现对掺杂型ZnS纳米材料光学性质及生物偶联功能的双重调节。最终,以这种掺杂型ZnS/Au纳米复合材料作为荧光标记材料实现人IgG等生物分子的高灵敏免疫分析。

中文关键词： ZnS；荧光；纳米复合材料；免疫检测；

英文摘要： Semiconductor nanocrystals have attracted significant attentions in biomedical fields during the past few years owing to their unique optical properties, such as tunable wavelength, narrow bandwidth, stability, and continuous excitations. The environmental safety and centralized material system are the main problems that limited the development of the fluorescent semiconductor biolabels. Therefore, the present project intends to obtain a kind of novel fluorescent nanocomposites for biolabeling and bioassay. The researches will start from the preparations of water-dispersive doped ZnS nanomaterials. The influences of the doping to the optical properties of the ZnS based nanomaterials will be studied. Then, doped ZnS/Au nanocomposites will be formed by using multi-sulfhydryl compound as link bridge. Utilizing the binding affinity between Au and biomolecules, the bioconjugations of the doped ZnS nanomaterials can be further improved. Simultaneously, the localized surface Plasmon resonance effect of the Au nanoparticles can be designed to enhance the fluorescence of the doped ZnS nanomaterials. Finally, sensitive immunoassays are expected to be realized based on the optimized doped ZnS/Au fluorescent nanolabels.

英文关键词： ZnS；fluorescence；nanocomposites；immunoassay；