对映-贝壳杉烷二萜介导拟南芥生长素极性运输的分子调控机制

项目名称： 对映-贝壳杉烷二萜介导拟南芥生长素极性运输的分子调控机制

项目编号： No.31460063

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 丁兰

作者单位： 西北师范大学

项目金额： 50万元

中文摘要： 迄今，已发现植物次生代谢产物20余万种，它们在植物竞争和植物群落构建中发挥关键作用。对映-贝壳杉烷二萜是香茶菜属植物最主要的次生代谢产物，分子构型丰富独特，目前对它们调控植物生长发育的机制知之甚少，尤其对介导植物生长素极性运输的分子机制还完全不知。植物生长发育依赖于生长素极性运输所建立的生长素浓度梯度，植物响应环境变化进行适应性生长与生长素极性运输密切相关，研究植物次生代谢产物介导生长素极性运输机制是揭示植物之间化感作用的核心问题，但报道极少。本研究利用Western Blotting、qRT-PCR 、激光共聚焦显微镜及高效液相分析等技术，采用系列拟南芥生长素极性运输载体蛋的转基因株系、突变体及生长素分子标记株系为研究平台，研究4种不同构型二萜对拟南芥幼胚和幼苗生长素极性运输蛋白的亚细胞定位、蛋白及基因表达的调控机制，揭示可能的靶基因，阐释该类化合物介导拟南芥生长素极性运输的分子机制。

中文关键词： 拟南芥；对映-贝壳杉烷二萜；生长素极性运输；生长素极性运输载体；分子机制

英文摘要： To date, more than 200 thousand plant secondary metabolites have already been described, which play a crucial role in plant competition and community establishment. Ent-kaurene diterpenoids are the main secondary metabolites in genus Isodon and have various specific molecular structures; nevertheless, little is known on mechanisms how they regulate plant growth and development. Particularly, the exact mechanisms of ent-kaurene diterpenoids on polar auxin transport are still unknown. Polar auxin transport is not only required for formation of auxin concentration gradients that is of importance for plant growth and development, but also is strictly related to adaptability growth in response to environment changes. Thus, studying mechanisms on plant secondary metabolites mediating polar auxin transport is the key to reveal plant-plant allelopathic interactions. Here in this project a research platform will be established by Arabidopsis thaliana transgenic line of auxin polar transport carrier proteins, mutants failed in influx or efflux carriers and auxin-mark transgenic line, meanwhile some techniques including Western Blotting, qRT-PCR, laser scanning confocal microscopy, high performance liquid chromatographic analysis and others will be applied. We focus on studying the mechenisim of four ent-kaurene diterpenoids on regulating subcellular localization and expression of six auxin polar transport carrier protein in hypocotyl and seedling of Arabidopsis thaliana, further to reveal their possible target genes and ultimately explain molecular mechanisms on ent-kaurene diterpenoids mediating polar auxin transport in Arabidopsis thaliana.

英文关键词： Arabidopsis thaliana;Ent-kaurene diterpenoids;polar auxin transport;auxin polar transport carrier;molecular mechanism