枸杞多糖对精原干细胞增殖、分化及移植的影响

项目名称： 枸杞多糖对精原干细胞增殖、分化及移植的影响

项目编号： No.30860282

项目类型： 地区科学基金项目

立项/批准年度： 2009

项目学科： 电工技术

项目作者： 马良宏

作者单位： 宁夏医科大学

项目金额： 25万元

中文摘要： 枸杞多糖（LBP）具有防辐射、抗氧化及缺氧、保护生殖系统等功能。LBP 联合精原干细胞（SSCs）移植为男性不育的治疗开辟新的方法，尤其对生精功能阻滞的无精子症患者具有独特的临床应用价值。我们在前期对SSCs 分离、纯化及移植研究的基础上，拟建立Sertoli 细胞饲养层，体外模拟SSCs 增殖与分化的微环境，采用LBP 或LBP 联合细胞因子促进SSCs 体外增殖与分化。建立化疗致不育动物模型，利用显微注射技术将SSCs 移植到受者曲细精管内，采用LBP 或LBP 联合细胞因子修复体内已破坏的生殖内分泌网络调控系统，促进SSCs 体内增殖与分化。在冷冻保存液的配方中加入LBP，观察SSCs 移植的效果，优化冷冻保存方法。研究内容：①BP 对SSCs 体外增殖与诱导分化的影响；②#36890;过SSCs 移植，观测LBP 对SSCs 体内增殖与分化的影响；③#20248;化SSCs 的冷冻保存液配方；④BP 促生精功能恢复机制的探寻。

中文关键词： 枸杞多糖；精原干细胞；增殖；分化；移植

英文摘要： Lycium barbarum polysaccharide(LBP) have the function of anti-radiation,anti-oxidation, anti-hypoxia and genital system protection etc.Combination LBP and spermatogonial stem cell transplantation techniques provide a new strategy for male infertility, which have an unique clinical potential especially for spermatogenesis arrested azoospermatism patients. Based on our previous research on spermatogonia isolation,purification and transplantation, we will establish Sertoli cell feeder layer and imitate the microenvironment of spermatogonia proliferation and differentiation in vitro in this study,and utilize LBP or combine with cytokine to induce proliferation and differentiation of spermatogonia in vitro.Establishing the male infertility animal model owing to chemotherapy and transplanting spermatogonial stem cells into the donor seminiferous tubuli by microinjection technique, we try to use LBP or cytokine to repair the destroyed reproductive endocrine system and promote spermatogonia to proliferate and differentiate in vivo. Add LBP to the cryopreservation solution,and evaluate the effect of spermatogonia transplantation. In this study,we use LBP to: (1) induce differentiation and proliferation of spermatogonia in vitro; (2) promote spermatogonia to differentiate and proliferate in vivo;(3) optimize the method for spermatigonia cryopreservation;(4) explore the functional recovery mechanism of spermatogenesis.

英文关键词： lycium barbarum polysaccharide;spermatogonial stem cell;proliferation;differentiation;transplantation