妊娠小鼠骨组织TRPV5和TRPV6的表达规律及其在胎盘钙转运中的作用探讨

项目名称： 妊娠小鼠骨组织TRPV5和TRPV6的表达规律及其在胎盘钙转运中的作用探讨

项目编号： No.31260630

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 李素华

作者单位： 西南林业大学

项目金额： 46万元

中文摘要： TRPV5和TRPV6 (transient receptor potential vanilloid type 5、6)是钙离子的高选择性通路蛋白，参与了骨代谢及胎盘钙转运过程中的钙离子跨细胞转运。本项目拟以妊娠期小鼠为研究对象，分析TRPV5、TRPV6蛋白在妊娠母体骨组织动态表达规律，监测同期骨代谢相关指标，揭示TRPV5/6在骨代谢中的作用；同时分析孕中后期胎儿胎盘TRPV5、TRPV6蛋白的动态变化，检测胎儿及胎盘脐静脉钙含量，探讨其与胎儿钙代谢的相关性，揭示胎盘与母体骨组织TRPV5/6的作用及其机制。此外，通过食物添加1,25-(OH)2D建立其高血清水平妊娠小鼠，研究1,25-(OH）2D对TRPV5/6在妊娠期钙代谢中的调控作用。藉此，阐明TRPV5和TRPV6在妊娠期钙代谢中的作用，完善孕期母体和胎儿的钙转运机制，为兽医临床筛选、纠正孕期钙负平衡提供实验依据。

中文关键词： ；TRPV6；钙转运；妊娠；胎盘

英文摘要： The transient receptor potential vanilloid 5 and 6 (TRPV5, 6) are calcium selective channels that are involved in the absorption and reabsorption of calcium across bone tissue cells and trophoblast cells. The project was designed to reveal the role of TRPV5/6 in the bone metabolism firstly by testing the expression dynamic law of the two proteins on the bone tissue of Kunming Mouse during the pregnancy, and by analyzing the relationship of the levels of protein contents and bone metabolism relevant indicators during the same period. Secondly, the team was intended to investigate the effect of TRPV5/6 on the placenta calcium transport. So the calcium content of the fetus and umbilical vein would be deterninated, as well as the contents of TRPV5/6 in the fetal placenta of mice in late pregnancy. By clearing the relation of TRPV5/6 content in the placenta and maternal bone tissue, we were expected to clear the relation of maternal bone metabolism and placenta calcium transport. In addition, high diet 1,25-(OH)2D level was used to induct high serum 1,25-(OH)2D level on the pregant mice on the clinical animal experiments, so as to investigate the regulating effect of 1,25-(OH)2D on calcium metabolism in pregnancy. On the whole, this program was to clarify the role of TRPV5/6 in calcium transporter and provide basis f

英文关键词： TRPV5；TRPV6；Calcium transport；Pregnancy；Placenta