苦蘵抗肿瘤活性成分（Physagulin R和Physalin H）的遗传基础解析和品种选育

项目名称： 苦蘵抗肿瘤活性成分（Physagulin R和Physalin H）的遗传基础解析和品种选育

项目编号： No.31470407

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 王慧中

作者单位： 杭州师范大学

项目金额： 85万元

中文摘要： 我国是人口大国，也是肿瘤病患高发的国家之一。传统中药材的有效活性成分分离和抗肿瘤新成分发掘已成为现代中药研究的热点。本项目旨在对最近研究发现苦蘵中具有抗肿瘤活性新成分Physagulin R和Physalin H的遗传基础进行解析，并服务于药材新品种选育。研究以农作物遗传研究思路为基础，以多个苦蘵F1分离群体和野生种质大样本群体为材料；利用高效液相分离技术获得准确的Physagulin R和Physalin H表型数据；利用转录组测序进行基因表达差异分析；参考同科近缘种番茄和土豆全基因信息，运用转录组和简化基因组测序获得各群体全基因组SNP标记；据此对上述成分含量的遗传基础进行连锁定位和全基因组关联分析。再通过候选基因关联分析，深入挖掘两种方法共同解析的Physagulin R和Physalin H含量相关基因内标签SNP位点，构建分子标记辅助育种体系，用于苦蘵种质资源筛选和新品种选育。

中文关键词： 苦蘵；抗肿瘤；遗传基础；候选基因关联分析；育种

英文摘要： China has the largest population in the world, and is one of the countries with high-incidence of cancer. As one of the origins of the TCM products, separation and exploration of the key components with anti-tumor efficacy from traditional Chinese medicinal materials has become a focus in modern Chinese medicine research. The suggested project aims at the genetic dissection of the newly-identified compositions of Physagulin R and Physalin H, which have high anti-tumor activity and were firstly discovered in our lab. The multiple F1 populations and a large sample of wild germplasm of Physalis angulata are to be tested with HPLC technology to establish the high throughput phenotypic data; the whole transcriptome sequencing is to be used in comprehensive evaluation of differential gene expression analysis for individuals of F1 populations; with the whole genome sequence information of close- related species of tomato and potato as reference, genome-wide SNP markers of the above populations is to be obtained on the basis of transcriptome sequencing and restriction-site associated DNA (RAD) sequencing; both QTL linkage mapping and genome-wide association analysis (GWAS) strategies are to be applied for genetic dissection. And also, the tag SNPS of Physagulin R and Physalin H related genes will be evaluated with candidate genes association mapping, and SNP marker assisted breeding system will be established in germplasm resources selection and new variety breeding of Physalis angulata.

英文关键词： Physalis angulata;antitumor;genetic basis;candicandidate gene association studies;breeding