甜玉米柔嫩度关联分析及基因定位

项目名称： 甜玉米柔嫩度关联分析及基因定位

项目编号： No.31471507

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 李小琴

作者单位： 华南农业大学

项目金额： 80万元

中文摘要： 本项目针对我国甜玉米品质性状柔嫩度遗传研究不足，用SSR等分子标记对甜玉米的重要品质性状-柔嫩度基因进行QTL定位与关联分析。在对本实验室自有的127份甜玉米自交系用显微测微尺和38份甜玉米自交系用果实硬度计法检测柔嫩度的基础上，选择柔嫩度差异大的两个甜玉米自交系A14和A26（sh2sh2）作亲本，构建RIL群体，参照前人玉米分子标记连锁图合成SSR引物，用亲本筛选多态性，对RIL群体进行DNA带型检测，构建分子标记连锁图，用WinQTL Cart等软件分析甜玉米柔嫩度的QTL，扫描主效QTL位点，在主效QTL区间加密SSR标记，选用QTL峰处SSR标记对自有的127份甜玉米自交系群体进行扩增并测序，进行群体结构分析及柔嫩度基因关联分析。本研究将为进一步探讨柔嫩度的遗传机理奠定坚实的基础，为柔嫩度主效QTL克隆、优质柔嫩度甜玉米育种提供重要依据并开发可用于分子标记辅助选择的关键分子标记。

中文关键词： 甜玉米；关联分析；柔嫩度；基因定位

英文摘要： The project focused on the research gaps of tenderness trait in sweet corn. QTL mapping and association mapping of tenderness will be done by SSR markers. Based on the detection of tenderness using 127 sweet inbred lines by micrometer and 38 sweet inbred lines by FHM (fruit hardness meter), A14 and A26（sh2sh2） with large difference of tenderness have been selected as parents to build mapping population of RILs. SSR primers has been selected by the published maize linkage map. The two parental lines will be used to screen SSR marker polymorphism. A linkage map will be constructed using SSR markers. The QTLs controlling tenderness will be mapped using WinQTL Cart and the main QTLs will be detected. More SSRs between the main QTL's interval will be used to fine map the main QTL. The SSRs located on the peak value will be selected to detect the 127 sweet inbred lines. The outcome of PCR will be sequenced to find single-nucleotide polymorphism (SNP) and the population structure analysis and association analysis were conducted. The results of this project will help us to understand the mechanism of tenderness in sweet corn. It is important to clone the genes controlling tenderness and to help breeding sweet corn variety with better tenderness. It will help to design key PCR markers to improve tenderness by MAS in sweet corn.

英文关键词： sweet corn;association mapping;tenderness;gene mapping