microRNA在缺血再灌注致急性肾损伤中的作用及机制研究

项目名称： microRNA在缺血再灌注致急性肾损伤中的作用及机制研究

项目编号： No.81470967

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 张文

作者单位： 上海交通大学

项目金额： 73万元

中文摘要： 缺血再灌注致急性肾损伤（AKI）是一个涉及到免疫炎症反应的病理过程，并可根据病程的变化分为始动、发展、修复阶段。我们前期通过对microRNA芯片分析认为在AKI进程中有一组microRNA参与各阶段并发挥作用。因此本项目拟用microRNA显著性水平及炎症程度阶段相关与否，筛查参与AKI病程的microRNA。采用mimic/inhibitor基因转染技术对其表达进行干预，观察炎症程度、细胞表型、动物预后等损伤及修复相关指标变化，确定何种阶段相关的microRNA可能成为潜在的治疗靶标。通过mRNA显著性、Luciferase技术、Pathway analysis等技术鉴定microRNA的靶基因并分析相关信号通路，进一步探究缺血性AKI的病理机制，为其防治提供新的策略。

中文关键词： 急性肾损伤；微小RNA；免疫活化；炎症反应；信号转导

英文摘要： Immune-inflammatory responses is involved in the progression of ischemic-reperfusion(I/R) kidney injury, which can be departed into three phases: initiation, development, repair. On the basis of our previous study of gene-chip analysis on microRNA, we hypothesized that a number of microRNA were involved in the process of AKI, and our aim was to screen the degree/stage-related microRNA through correlation analysis with inflammation. In order to make the optimal choice of therapeutic target, we will take the form of gene modification to intervene in all the degree/stage-related microRNA and evaluate the degree of inflammation,changes of cell phenotype, prognosis of animal etc.so as to obtain innovative therapeutic targets. considering the exploration of pathogenesis in I/R induced AKI model was of great importance, not only would we analyze the target gene(mRNA) by expression level of mRNA, luciferase, we also intend to make mRNA-related pathway analysis, which could provide strategic reference for prevention and treatment of AKI.

英文关键词： Acute kidney injury;MicroRNA;Immune activation;Inflammation response;Signal transduction