近红外II区荧光成像技术辅助的病毒纳米颗粒活体肿瘤靶向研究

项目名称： 近红外II区荧光成像技术辅助的病毒纳米颗粒活体肿瘤靶向研究

项目编号： No.31470931

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 李峰

作者单位： 中国科学院武汉病毒研究所

项目金额： 92万元

中文摘要： 病毒纳米颗粒（VNP），是由病毒衣壳蛋白组装形成的典型尺寸20-200 nm的空心球或管，近年来成为发展智能药物载体、活体探针、组织工程材料、多功能诊疗平台的热门天然纳米材料。如何有效的到达目标部位是VNP活体生物医学应用的一个关键问题，而目前对VNP活体分布行为与其表面性质关联性的认识还十分有限。Ag2S量子点是一种新型近红外II区荧光纳米探针，提供空前的活体成像性能。本项目针对最受关注的肿瘤靶向问题，在建立基于Ag2S包装的VNP活体成像方法的基础上，结合其他手段，系统研究不同的聚乙二醇和靶向分子共修饰模式对VNP肿瘤靶向性的影响，试图在前者的屏蔽免疫清除与后者的肿瘤识别功能之间找到平衡，以期显著提高VNP的靶向效率。本项目将为蛋白纳米结构的活体行为研究提供新的方法和思路，揭示VNP活体行为与表面性质之间的关联性规律，也为此类材料生物医学应用的突破奠定基础。

中文关键词： 纳米生物材料；量子点；纳米载体；肿瘤靶向；病毒纳米颗粒

英文摘要： Viral nanoparticles (VNP) are hollow spheres or tubes typically 20-200 nm in size assembled from viral capsid proteins. VNP has been extensively recognized as a category of natural nanomaterials for developing intelligent drug carriers, probes for in vivo imaging, tissue engineering materials, multifunctional theranostic platforms, etc. in recent years. Effective accumulation of VNP at the site of interest in the living body is a critical step in most in vivo biomedical applications of VNP. However, how the surface properties of VNP impact its in vivo distribution and behaviors remains to be elucidated. Ag2S quantum dot (QD), a new kind of nanoprobe with bright near-infrared II (NIR-II) fluorescence, can offer unprecedented performance in in vivo optical imaging. This proposal, which takes in vivo tumor targeting, one of the hottest topics in nanomedicine as a model, will systematically investigate the effects of varied surface co-modification patterns with polyethylene glycol (PEG) and tumor targeting ligands on in vivo distribution and the tumor-homing capability of VNP, with the aid of Ag2S-encapsulation-based NIR-II imaging technique, which is being developed in our lab. We will try to balance the immune shielding of PEG and the tumor recognization of targeting ligands, so that a significantly enhanced tumor-homing efficiency of VNP can possibly be achieved. The project will provide novel methodology and insights to the in vivo studies of protein nanostructures, reveal the rules how surface properties of VNP affect its in vivo behaviors, and pave the way for the breakthrough of nanomedicine enabled by such kind of materials.

英文关键词： bionanomaterial;quantum dot;nano carrier;tumour targeting;viral nanoparticle