可激活核酸适配体磁共振成像分子探针在肿瘤细胞成像中的应用

项目名称： 可激活核酸适配体磁共振成像分子探针在肿瘤细胞成像中的应用

项目编号： No.21305120

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 李菁菁

作者单位： 徐州医学院

项目金额： 25万元

中文摘要： 在对疾病及疾病标志物甄定与检测的众多分析方法中，发展与临床应用密切相关的分子成像技术具有重要意义。磁共振分子成像利用磁共振成像（MRI）技术对体内特定分子进行成像，实现对病变早期、特异性诊断与疗效监测。其中，分子成像探针的设计与制备居于核心地位。研究较多的靶向性MRI分子探针存在的高本底背景、成像对比度不高以及灵敏度有限等问题使得可激活MRI分子探针逐渐成为未来的研究趋势。然而，目前该类探针的应用报道较少，研究对象范围相对狭小，并无涉及细胞和活体水平的MRI检测报道，在向临床应用转化过程中还需进行一系列深入研究。基于此，本项目拟发展微波多元醇法制备PEG-Gd2O3作为造影剂，分别以人乳腺癌细胞表面MUC1蛋白及活细胞内肿瘤相关C-myc mRNA为诊断对象，利用适配体的靶向识别与细胞内化能力、磁弛豫开关效应设计制备可激活核酸适配体MRI分子探针，实现肿瘤细胞磁共振分子成像检测应用。

中文关键词： 分子成像；纳米探针；磁共振成像；可激活纳米探针；荧光成像

英文摘要： Although various diagnostic and analytical detection methods for disease and disease-related biomarkers are currently available, developing molecular imaging techniques closely related to clinical applications is still in demand. Magnetic resonance molecular imaging is a type of technique utilizing MRI to image specific biomolecules in the body, which could achieve early prognosis of disease and monitoring of the therapeutic effect after treatment. The design and preparation of molecular imaging probe is the key to this technique. However, high background, low image contrast and limited sensitivity of the most studied targeting MRI molecular probe hampered wider applications of this technique. Activatable MRI molecular probe has great potential in future research. However, very limited applications of activatable MRI molecular probe have been reported so far and the scope of the targets is narrow. To our knowledge, no activatable MRI molecular probe has been developed for cellular or in vivo applications. Thus, more research should be conducted to better transfer this technique for clinical applications. In this project we plan to develop microwave-assisted polyol method to prepare PEG-Gd2O3 nanoparticles as contrast agent and propose that by designing smart activatable aptamer MRI molecular probes which integr

英文关键词： molecular imaging；nanoprobe；magnetic resonance imaging；activatable nanoprobe；fluorescence imaging