手性三唑类农药对爬行动物性腺作用轴的内分泌干扰效应及分子机制研究

项目名称： 手性三唑类农药对爬行动物性腺作用轴的内分泌干扰效应及分子机制研究

项目编号： No.21477152

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 化学工业

项目作者： 王会利

作者单位： 中国科学院生态环境研究中心

项目金额： 86万元

中文摘要： 爬行动物作为生态系统中重要组成部分，其种群数量呈下降趋势。研究发现农药使用造成内分泌干扰所导致的繁殖力下降可能是其种群密度降低的原因之一。三唑类农药是目前我国使用量最大的一类具有内分泌干扰效应的手性杀菌剂，其对爬行动物的内分泌干扰效应尚无相关报道。本项目拟以中国本土物种--丽斑麻蜥为研究对象，探讨三唑类农药对爬行动物的内分泌干扰效应及其分子机理。通过三唑类农药不同对映体的暴露，从生理生化角度探讨三唑类农药对丽斑麻蜥性腺作用轴的内分泌干扰效应，明确敏感的检测终点及相应的剂量-效应关系；从基因水平上阐明三唑类农药对下丘脑-垂体-性腺作用轴的影响，明确其分子机制；通过三唑类农药不同对映体在丽斑麻蜥体内的代谢富集规律、生理生化指标、组织损伤效应以及基因表达分析等综合评价三唑类农药对爬行动物的生态毒理学效应。本研究将为农药的生态和健康风险评价提供科学依据，并为保护我国本土物种提供毒理学数据。

中文关键词： 手性三唑类农药；爬行动物；性腺作用轴；分子机制；生态毒性

英文摘要： Reptile is one of the important parts in ecosystem, however its population is declining. Studies showed reptiles' fecundity decline caused by the endocrine disruption due to pesticide application was one of the main reasons. Triazole pesticide, whose application amount in our country is the highest currently, is a class of chiral fungicide with endocrine disrupting effects. But no information was reported on its endocrine disrupting effects on reptiles. In this study, with the Chinese native reptile species-Liban lizard (Eremias argus) being as the research object, the endocrine disrupting effects of the typical triazole fungicides on the reptiles and the molecular mechanism of action will be studied. First, through exposing to the different enantiomers of triazol fangicides, their endocrine disrupting effects on gonad axis of Eremias argus in the level of physiology and biochemistry will be studied, so as to determine the sensitive detection end points and the corresponding dose-effect relationship. Second, the impact of the triazole pesticide on hypothalamic-pituitary-gonadal axis in the genetic level will be studied, so as to disclose the molecular mechanisms of action. Third, the potential ecological risks of the triazole fungicide on reptiles will be evaluated by integrateing the metabolic and enrichment characteristics of different enantiomers in Eremias argus, physiological and biochemical index, effect of histological damage, and the genes expression profiling etc. This project aims to provide science basis for the risk assessment of pesticides on ecology and health, and toxicological data in conserving our local reptile species.

英文关键词： chiral triazole pesticides;reptile;HPG;molecular mechanism;ecotoxicity