水通道蛋白MR分子成像（AQP MR）无创性早期诊断肝脏纤维化的实验及临床研究

项目名称： 水通道蛋白MR分子成像（AQP MR）无创性早期诊断肝脏纤维化的实验及临床研究

项目编号： No.81471718

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 郭启勇

作者单位： 中国医科大学

项目金额： 72万元

中文摘要： 肝纤维化是肝炎发展至肝硬化的可逆性阶段，肝纤维化的早期诊断对肝脏纤维化疗效提高，以及对肝硬化及肝癌的防治至关重要。但目前缺乏安全可靠、早期精准定量化、可行性强，以及能够在临床普及使用的肝脏纤维化早期诊断分子影像方法。郭启勇教授的研究组发现肝纤维化大鼠模型在肝脏纤维化早期（S1期）水通道蛋白表达特异性增高，并且能够采用新型MR分子成像技术无创性检测。本研究在已经取得的基础和临床预实验研究成果基础上，计划进一步深入水通道蛋白磁共振分子成像技术(AQP MR)大鼠动物实验研究,并将AQP MR技术推向临床研究。依据常规病理分期和AQP(1、3、4、7、8、9)亚型定量表达结果，将AQP MR技术一次扫描获得的肝组织血流灌注、水分子在肝组织扩散和肝组织细胞AQP的表达定量化数据，结合MR常规扫描和相应的血清学指标，达到对肝脏纤维化S1、S2期早期诊断目的，创建AQP MR早期肝纤维化诊断方法。

中文关键词： 磁共振成像；分子影像学；扩散加权成像；水通道蛋白

英文摘要： Liver fibrosis is the reversible stage from hepatitis to liver cirrhosis, the early diagnosis of which plays important role in improving the curative effect of liver fibrosis, and preventing liver cirrhosis and liver cancer. However, at present, there is lack of safe, reliable, early, accurate, quantified, and feasible molecular imaging method, which can wide spread in clinical use. Professor Qiyong Guo 's team found that the expression of water channel protein in rats model of early liver fibrosis (S1) increases specifically, and it can be noninvasively detected with the new MR molecular imaging technique. This study has been made on the basis of the fundamental and clinical preliminary experimental research, plan to study thorough the animal research of water channel protein MR molecular imaging technology (AQP MR), and spread this technology into clinical research. Based on routine pathological staging and the quantitative expression of AQP (1, 3, 4, 7, 8, 9) subtype, quantify the data of AQP MRI scan, including blood perfusion, diffusion of water molecules in the liver tissue and expression of AQP on cell membrane, and combining with routine MR scanning and corresponding serological indexes, realize the goal of early diagnosis of liver fibrosis in S1, S2 stages, create AQP MR method in early diagnosis of liver fibrosis.

英文关键词： magnetic resonance imaging;molecular imaging;diffusion weighted imaging;Aquaporin