可早期实时监测循环中转移性肿瘤细胞的在体流式图像细胞仪

项目名称： 可早期实时监测循环中转移性肿瘤细胞的在体流式图像细胞仪

项目编号： No.61227017

项目类型： 专项基金项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 魏勋斌

作者单位： 上海交通大学

项目金额： 280万元

中文摘要： 本项目旨在研制在在体水平上用于生命科学基础研究的光学仪器“在体流式图像细胞仪”。该仪器在高速影像和体外流式细胞仪的基础上创新性地实现在体、实时、动态、无损监测小动物血循环内的荧光标记细胞(如循环肿瘤细胞)，并定量分析。项目拟解决在面上基金资助下发展起来的“活体流式细胞计数新方法”在仪器化方面的技术难点。改进现有光路，提高检测的信噪比；增加488nm 激发通道，实现对不同类型的细胞监测并定量分析；实现整个装置的科学仪器化；使用小波滤波和C++算法，提高计数准确率；并实现血流流速的在体分析。前期工作被国际肿瘤权威刊物Cancer Research接受并评价为突破性技术。目前基于荧光的在体流式图像细胞仪只能检测较为表浅的小血管, 项目拟通过与光声检测和内窥镜技术相结合,研制“在体光声流式细胞仪”，实现深层大血管检测，提高循环肿瘤细胞的检测灵敏度。并研究循环肿瘤细胞的在体无标记光学定量检测。

中文关键词： 生物医学光学方法；流式细胞仪；在体荧光成像；循环肿瘤细胞；信号分析

英文摘要： This project is aimed to develop a novel biomedical optical instrument, in vivo imaging flow cytometry, to be applied to in vivo research of basic life science. The proposed instrument combines the techniques of high-speed imaging and conventional flow cytometry to realize dynamic and non-invasive monitoring of fluorescently labeled cells in circulation of small animals in real time, as well as quantitative analysis. We will focus on solving technical difficulties for instrumentation of "new cell counting method of in vivo flow cytometry" funded by NSFC project. We will optimize current optical layout, increase signal-to-noise ratio of detection system; add a new channel of 488 nm excitation to discriminate different cell types and analyze quantitatively; realize scientific instrumentation for the whole system; improve existing software by introducing wavelet-based algorithm and C++ programming to increase counting accuracy; obtain the velocity parameter of blood flow in vivo. Considering that fluorescence-based in vivo imaging flow cytometry can be only applied to small and superficial vessels, this project is going further to add photo-acoustic detection and endoscopic technique to develop in vivo photo-acoustic flow cytometry to detect deep and large vessels to enhance detection sensitivity of circulating tum

英文关键词： biomedical optical method；flow cytometry；in vivo fluorescence imaging；circulating tumor cells；signal processing