典型农药残留的双重信号增强SPR免疫传感检测技术研究

项目名称： 典型农药残留的双重信号增强SPR免疫传感检测技术研究

项目编号： No.21207161

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 环境化学

项目作者： 彭媛

作者单位： 中国人民解放军军事医学科学院

项目金额： 25万元

中文摘要： 农药残留是造成环境污染、危害食品安全的重要因素之一，加强环境中农药残留检测，有利于保障人民身体健康、促进相关产业健康发展。但农药残留样品往往基质复杂、含量极低，高灵敏检测技术联合信号放大技术是实现环境中痕量农药残留快速检测的重要手段。本研究拟以多种典型农药（对硫磷、阿特拉津、毒死蜱等）为靶标，以SPR传感器件为换能器，磁性纳米微球-抗体偶联物为前处理分离富集材料和一级传感信号增强元件，在传感器芯片表面制备高生物相容性的壳聚糖膜，以固定抗原并提高芯片贮存稳定性，同时在免疫检测体系中引入SPA作为二级放大传感元件，构建集分离、富集和检测于一体的磁性微球-SPA双重信号放大SPR免疫传感系统，实现环境和食品中农药残留的痕量检测。该项目研究成果可用于环境监测、食品安全和生物工程等领域，推动相关学科的分析检测技术发展，具有重要的理论意义和广阔的应用前景。

中文关键词： 农兽药残留；信号增强；免疫传感检测；表面等离子体共振；

英文摘要： Residual pesticides are used to be considered as one of the most important pollutants in environment and foods. Signal enhanced detecting technology with high sensitivity is exigently required, for the reason that target pollutants are usually contained by samples with complex matrix.The object of this research is to establish a double enhanced surface plasmon resonance (SPR) immunosensing system for rapid determination of typical pesticide residues (parathion, atrazine, ractopamine, etc.). The targets are separated and enriched by magnetic nanospheres-antibody conjugates which are also performed as the sensitive recognize element and primary signal enhanceing material. Membrane of high biocompatibility material (chitosan etc.) is prepared on SPR chip surface to immobilize the antigen and increase the stability of the modified sensor chip. Staphylococcal protein A (SPA) is introduced into the detecting system as a secondary signal enhanceing material. A immunoseneing system with double signal enhancement will be established, which can be used to detect trace amount pesticides in environment and food samples. The results of this research can be utilized in environment pollutants monitoring, food safety and bioengineering, and will promote the development of relative subject analyzing techniques. Furthermore, good

英文关键词： pesticide and veterinary drug residue；signal enhancement；immunosensing detection；surface plasmon resonance；