高通量高灵敏度等离激元共振增强OI-RD光学生物传感方法及应用研究

项目名称： 高通量高灵敏度等离激元共振增强OI-RD光学生物传感方法及应用研究

项目编号： No.61505032

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 无线电电子学、电信技术

项目作者： 费义艳

作者单位： 复旦大学

项目金额： 23万元

中文摘要： 生物分子相互作用的检测是生命科学领域研究的重要内容。药物筛选、组学研究等需要高通量、高灵敏检测生物分子相互作用，现有无标记检测仪器和方法还不能充分满足研究要求。斜入射光反射差法（OI-RD）是一种光学生物传感方法，能够高通量、无标记、实时检测上万个生物分子相互作用，但检测灵敏度有待进一步提高。本项目将采用表面等离激元共振增强效应来提高OI-RD的灵敏度。将通过研究表面等离激元共振增强OI-RD技术、系统分析噪声来源及其对检测极限的影响等，研究同时具备高通量（同时检测>10,000个生物分子）和高灵敏度（检测极限10^(-7)RIU，Refractive Index Unit）的无标记光学生物传感方法，为药物筛选、组学研究等领域提供新型生物学检测仪器。

中文关键词： 光学生物传感器；生物芯片；表面等离激元传感；高通量

英文摘要： Detection of biomolecular interactions is important for the deep understanding and fast development of life sciences. High-throughput and high-sensitivity detection of biomolecular interactions, which is required for the development of drug screening and omics studies, can’t be fully satisfied by currently available label-free instruments. OI-RD (Oblique-Incidence Reflectivity Difference), one of optical biosensors, provides high-throughput label-free in-situ and real-time characterization for biomolecular interactions. While the sensitivity of OI-RD needs to be increased greatly to meet requirements of drug screening and omics studies. We propose to increase OI-RD sensitivity based on enhancement effect of surface plasmon resonance. Through the study of surface plasmon resonance enhanced OI-RD and sources of noise and their effects on OI-RD detection limit, we will develop a novel optical biosensor, providing both high-throughput (simultaneous detection of >10,000 biomolecular interactions) and high-sensitivity (detection limit 10^(-7)RIU, Refractive Index Unit), which will provide novel solutions for drug screening and omics studies.

英文关键词： optical biosensor;biochip;surface plasmon resonance sensing;high-throughput