黄瓜ERF转录因子CsERF1调控耐涝性的分子机理研究

项目名称： 黄瓜ERF转录因子CsERF1调控耐涝性的分子机理研究

项目编号： No.31201640

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 齐晓花

作者单位： 扬州大学

项目金额： 21万元

中文摘要： 黄瓜根系入土浅、通气组织不发达、再生能力差，因此极易受到涝胁迫危害。前期通过数字基因表达谱筛选获得应答涝胁迫的ERF转录因子，并进一步获得了该基因参与调控黄瓜耐涝性的初步证据。本项目拟通过构建CsERF1基因的过量表达株和RNAi抑制表达株，采用涝胁迫与乙烯作用抑制剂1-MCP、乙烯作用促进剂乙烯利预处理相结合，比较转化植株和野生型之间耐涝性（涝胁迫响应标志基因和形态性状的变化）差异；分析外源乙烯对CsERF1基因及黄瓜耐涝性的影响；研究转化植株和野生型在三种处理下植物内源乙烯和生长素含量变化及相关基因的表达变化，同时结合不定根形成和根系细胞凋亡情况，阐明黄瓜涝胁迫过程中CsERF1基因诱导的形态改变与激素变化之间的联系；最终明确CsERF1基因缓解黄瓜涝胁迫的分子机制。CsERF1基因功能的解析不仅对黄瓜耐涝种质资源创新具有重要意义，也为完善ERF转录因子信号转导机制提供重要信息。

中文关键词： 黄瓜；不定根；乙烯；生长素；活性氧

英文摘要： Cucumber (Cucumis sativus L.) is easily affected by heavy rain and subsequent periods of soil flooding in summer. In previous research, the gene expression profiling of cucumber plant over a period of waterlogging treatment has been analyzed，and ethylene responsive factor was found to be involved in waterlogging response. The aim of our project was to identify the function of CsERF1. Therefore, the CsERF1 over-expression and RNAi transformation plant will be constructed. Waterlogging, waterlogging with 1-MCP, waterlogging with ethylene pretreatment will be applied to check the CsERF1, ADH、PDC、SUS gene expression. The phenotype change of the transformed plant and the wild type under thredd treatments will be checked. The contents of ethylene and auxin, and gene involved in the synthesis and signal transduction of ethylene and auxin will be analyzed by qRT-PCR. Finally, we want to find the three following points. 1) Whether the gene expression of CsERF1 was regulated by ethylene or not? 2) How does the CsERF1 regulate ADH、PDC、SUS gene expression? 3) How does hormonal interplay during the phenotype change in waterlogged cucumber plant? The outcome of this study will help us to accelerate the cucumber genetic resource innovation of waterlogging resistance, and provide new insights of ERF signal transduction mechanis

英文关键词： Adventitious root formation；Auxin；Cucumber；Ethylene；Reactive oxygen species