基于代谢组学及热分析技术的地黄炭止血“存性”与制炭工艺相关性研究

项目名称： 基于代谢组学及热分析技术的地黄炭止血“存性”与制炭工艺相关性研究

项目编号： No.81673601

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 医药、卫生

项目作者： 张朔生

作者单位： 山西中医药大学

项目金额： 25万元

中文摘要： 中药炮制过程中有关“火力”、“火候”、“存性”等术语的表述与判定大多依然停留在经验判断基础上，缺乏客观化、数据化的标准，在很大程度上制约着本学科的发展；对中药饮片炮制过程中体外化学物质变化规律进行研究的思路与方法，不能完全反映中药饮片炮制前后功效变化的整体性与复杂性。本项目以生地炭“存性”差异为切入点，提出“地黄炭止血'存性'的物质基础，不仅包括体外转化产物，还包括体内代谢产物；而这些物质基础的量变与质变一定程度上主要取决于制炭工艺”的工作假说。通过采用代谢组学技术，结合热分析技术，对生地黄制炭（炒炭、煅炭）过程中药效物质变化过程进行系统研究，并对工艺参数进行量化表征；对生地黄炭在体内动态变化过程和代谢产物变化进行比较分析，进而阐明生地黄制炭（炒炭、煅炭）工艺与其止血“存性”之间的相关性，构建基于热分析-代谢组学技术的中药炮制工艺与炮制机制研究的新模式。

中文关键词： 地黄炭；代谢组学；热分析技术；止血存性；制炭工艺

英文摘要： About the terms of processing traditional Chinese material media such as firing strength, duration and degree of heating, nature of the drug partly preserved after carbonization, It is still mostly based on experience judgement, and lack of objective, numerical standards for the description and determination,to a great extent, restricts the development of this discipline. The traditional research idea and method chiefly focus on the variation about chemicals in vitro of processed traditional Chinese material media, it can not fully reveal the integrity and complexity of the efficacy changes before and after processed. Based on the breakthrough point that the effects of partly preservation of the nature of Dried Rehmanniae Radix Carbonisata is different from Rehmanniae Radix Praeparata Carbonisata,we put forward the working hypothesis that the effects of hemostasis ＆partly preservation of the nature of Rehmanniae Radix Carbonisata depend on its material baisis concluding transformation products in vitro & metabolites in vivo. The quantitative and quanlitative change of the material baisis to a extend mainly depend on the charcoal processing crafts .Drug metabonomics technology combined with thermal analysis technology will be used to systematically study the change progress of effective substance in the process of Charcoal Processing (Carbonization by Stir-Heating or by Calcining) of Rehmanniae Radix, to quantify the process parameters; and to analyze the dynamic process and metabolites in vivo about Rehmanniae Radix and Rehmanniae Radix Carbonisata, which will further illustrate the correlation between the charcoal processing crafts ( carbonization by stir-heating or by calcining ) and the effects of hemostasis ＆partly preservation of nature of Rehmanniae Radix, and establish a new research model of processing crafts and processing mechanism of TCM based on the technology of thermal analysis and metabonomics.

英文关键词： Rehmanniae Radix Carbonisata;metabonomics;thermal analysis technology; effects of hemostasis ＆partly preservation ;charcoal processing crafts