壮医药线点灸对佐剂关节炎大鼠NF-κB/IκB信号通路的影响

项目名称： 壮医药线点灸对佐剂关节炎大鼠NF-κB/IκB信号通路的影响

项目编号： No.81360571

项目类型： 地区科学基金项目

立项/批准年度： 2014

项目学科： 医药、卫生

项目作者： 蒋耀平

作者单位： 广西中医药大学

项目金额： 48万元

中文摘要： 类风湿关节炎是常见风湿性疾病，早期易出现关节骨破坏而致残，给患者和家庭带来极大危害和沉重经济负担。虽然早期联合使用慢作用药治疗取得一定的疗效，但仍有很多患者不可避免出现关节骨破坏和畸形。生物制剂成为近年治疗类风湿关节炎新途径，但随着此类药物推广运用，继发感染（特别是结核）等副作用也逐渐增多。药线点灸是广西民间传统的壮医外治疗法，具有简、便、廉和副作用小的优点，且疗效确切。本项目在观察壮医药线点灸对类风湿关节炎患者TNF-a、IL-1β影响基础上，运用实时荧光定量PCR技术、蛋白质印迹法检测壮医药线点灸治疗后佐剂关节炎大鼠NF-κB、IκB、TNF-αmRNA、IL-1βmRNA、COX-2mRNA表达变化，并用流式细胞仪和免疫组化法观察大鼠滑膜细胞凋亡。探讨壮医药线点灸治疗类风湿关节炎的作用机制和关键靶点，为壮医药线点灸疗法的推广提供理论基础，进一步完善壮医理论体系。

中文关键词： 壮医药线点灸;佐剂关节炎;NF-κB;IκB;细胞凋亡

英文摘要： Rheumatoid arthritis is a common rheumatic diseases, prone articular bone destruction and disability on early stage,causing great harm to the patients physical and mental health,being a heavy financial burden to families and society.Although the early joint use of slow-acting drugs has certain effect,but there are still many patients inevitably with articular bone destruction and deformity.In recent years, biologics become new ways of treatment of rheumatoid arthritis,however, with the use of these drugs to promote,secondary infection (especially tuberculosis) and other side effects also gradually increased.Medicated thread moxibustion is a folk External treatment of Zhuang medicine in Guangxi,having advantages of simple, convenient, inexpensive, little side effect and efficacy.On the basis of observing the impact of Medicated Thread Moxibustion of Zhuang Traditional Medicine treatment on rheumatoid arthritis patients with TNF-a, IL-1β,this project use real-time fluorescence quantitative PCR technology and Western blot to detect the expression changes before and after the treatment on adjuvant arthritis rats in NF-κB、IκB、TNF-αmRNA、IL-1βmRNA and COX-2mRNA expression in the synovial tissuee,and apoptosis of synovial cells are also observed using flow cytometry and immunonhistochemistry methods. Explore the mechanism of action and key targets of Medicated thread moxibustion Zhuang Traditional Medicine treatment medicine theory system to provide a theoretical basis for the promotion of Zhuang medicine medicated thread moxibustion therapy, and further improve Zhuang medicine theory system.

英文关键词： Medicated Thread Moxibustion of Zhuang Traditional Medicine;Adjuvant arthritis;NF-κB;IκB;Apoptosis