1,3-甘油二酯酶解产物在小肠上皮细胞中再酯化途径研究

项目名称： 1,3-甘油二酯酶解产物在小肠上皮细胞中再酯化途径研究

项目编号： No.31201343

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 膳食与营养

项目作者： 徐同成

作者单位： 山东省农业科学院

项目金额： 21万元

中文摘要： 1,3-甘油二酯（DAG）是天然油脂组成成分，与甘油三酯（TAG）相比，其摄入可以显著改善机体血脂代谢。1,3-DAG与TAG结构的差异，导致其水解产物在小肠上皮细胞中再酯化途径及代谢产物的差异，是其发挥健康功效的基础。诸多学者推测，1,3-DAG水解产物在小肠上皮细胞中可能通过磷脂酸途径重新酯化生成TAG，尚未得到证实，限制了对其作用机理的研究。 本项目以SD 大鼠为受试对象，应用膳食1,3-DAG 干预手段，研究小肠不同部位脂类组成的变化规律；采用酶活测定方法，探讨小肠粘膜中磷脂酸合成途径相关酶活性的变化趋势；利用分子生物学技术，阐明磷脂酸合成途径相关酶编码基因表达量的变化动态。通过上述研究，对探明1,3-DAG 在小肠内的酶解途径，以及酶解产物在小肠上皮细胞中的再酯化途径，对揭示其发挥健康功效的物质基础及潜在的作用机理具有重要意义，为研发具有血脂代谢调节功能的新型油脂提供理论支持。

中文关键词： 1;3-甘油二酯；磷脂酸途径；再酯化；糖尿病肾病；生长转化因子

英文摘要： 1,3-diacylglycerol (1,3-DAG) is one of the composition of natural oil. Compared with triacylglycerol (TAG), 1,3-DAG can improve the lipid metabolism. The diffirence in stucture between 1,3-DAG and TAG, accompanying with the difference of re-esterification pathway of metabolic hydrolysis products, was reagarded as the foundation of its health benefits. Many scholars speculated that the metabolic hydrolysis products of 1,3-DAG re-esterificated to TAG through phosphatidic acid synthesis pathway, but was not verified which limited the study on its benefits mechanism. In this study, SD rats will be took as tested animal, 1,3-DAG intervention will be applicated to study the viriation of lipids composition at different part of small intestine; Metabolic activity detection techniques will be applicated to study the activity viriation of enzymes related with phosphatidic acid synthesis pathway; Molecular biology techniques is applicated to study the expression viriation of encoding genes related with phosphatidic acid synthesis pathway. So, this study is very important to prove the enzymatic hydrolysis pathway and the re-esterification pathway of 1,3-DAG in the small intesine, reveal the material basis and mechanism of benefits of 1,3-DAG, and provide theoritical supports for the development of new functional oil with s

英文关键词： 1;3-diacylglycerols；Phosphatidic acid synthesis pathway；Re-esterification；diabetic nephropathy；TGF-β1