真菌云芝-F21a高效消除蓝藻水华的分子机制研究

项目名称： 真菌云芝-F21a高效消除蓝藻水华的分子机制研究

项目编号： No.51309003

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 水利工程

项目作者： 韩国民

作者单位： 安徽农业大学

项目金额： 25万元

中文摘要： 蓝藻水华的爆发严重破坏了水生态环境，给人们的生活带来了很大危害。真菌菌丝体包裹活体藻细胞并将其完全分解是治理藻类污染的一种新方法，但此种除藻模式的分子机制了解甚少。本项目以前期获得的一株高效去除蓝藻的真菌--云芝-F21a (Trametes versicolor)为供试菌，拟通过对其降解藻细胞过程中的真菌转录组进行测序、注释及比对分析，筛选获得差异表达显著的基因，预测关键代谢途径，并分析消除藻细胞过程中分解酶系组成及其变化；拟通过对酶系主要候选基因分别进行过量表达和RNAi抑制表达，分析分解酶系组成与除藻效率之间的关系；在此基础上，再结合影响真菌降解蓝藻效率的主要环境因子，确定影响真菌溶藻效率的关键分解酶。本项目研究结果，将为进一步了解真菌-蓝藻间相互作用机制、为除藻菌株改造提供理论依据。

中文关键词： 云芝F21a；消除蓝藻；关键分解酶系；关键代谢途径；分子机制

英文摘要： Outbreaks of cyanobacterial blooms seriously affected ecology and environments of water bodies, and were harmful to our dayly life. Recently, a new way of control algal blooms that fungal mycelia directly enwrap the algal cells and then degrade the cells was discovered. However, we know little about its molecular mechanism. In this project, a high efficiency algicidal fungus Trametes versicolor -F21a will be used as the tested strain. The transcripts of the fungal mycelia during the processes of eliminating algal cells will be sequenced, annotated and compared. Statistical significant different expressed genes will be selected and used to predict the key metabolic pathway. The components of the degradative enzymatic system will be elucadated according to the information of the fungal transcripts. The degradative system will be verified by overexpression and downregulation of key candidate genes. Combined with the major environmental factors, the correlation between the compositions of the system and efficencies of removal algal cells will also be calculated. The key decomposition enzyme that affect the efficiency of removal algal blooms will be confirmed in the end. The results of this project will not only enrich our knowledge of the interection between fungus and cyanobacterium, but also supply theory for the

英文关键词： T. versicolor -F21a；Eliminating algal cells；Key decomposition enzymes；Key metabolic pathways；Molecular mechanism