大庆湿地噬藻体和蓝藻的遗传多样性及其与环境因子的关系

项目名称： 大庆湿地噬藻体和蓝藻的遗传多样性及其与环境因子的关系

项目编号： No.31300425

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 荆瑞勇

作者单位： 黑龙江八一农垦大学

项目金额： 23万元

中文摘要： 噬藻体是一类分布极为广泛且能感染蓝藻的病毒，在调节浮游生物的丰度、群落结构以及生物地球化学循环中具有重要作用，而成为目前微生物学领域的一大研究热点。本项目拟以大庆天然湿地和人工湿地中噬藻体和蓝藻为研究对象，利用分离培养、PCR-DGGE及克隆技术，对噬藻体和蓝藻的遗传多样性及其分布特征进行研究；对噬藻体和蓝藻多样性在时间上的变化规律进行研究；应用PCA和CCA方法分析湿地环境因子与噬藻体和蓝藻多样性的关系。以此来阐明大庆湿地噬藻体和蓝藻的群落结构组成、系统发生及分布特征，揭示环境因子对噬藻体和蓝藻多样性的影响，为研究噬藻体与蓝藻间相互关系、明晰噬藻体在湿地生态系统中的结构与功能、开发治理水华的微生物资源提供重要的基础资料。

中文关键词： 大庆湿地；蓝藻；噬藻体；多样性；

英文摘要： Cyanophage was a kind of virus infecting cyanobacterium and distributed extensively. The research on cyanophages was a hot spot due to its playing a significant role in regulating the abundance of cyanobacterial plankton, community structure and in biogeochemical cycle. In the project, the genetic diversity and distribution of cyanophages and cyanobacteria in the natural and constructed wetlands in Daqing were investigated and the temporal change of diversity of cyanophages and cyanobacteria in the Daqing wetlands were also done with the molecular biological methods of PCR-DGGE and clonging combined with traditional isolation and purification; the PCA and CCA were applied to analysis the relationship among the environmental factors, diversity of cyanophages and cyanobacteria.Through this study, the community composition, phylogeny and distribution characteristics of cyanophages and cyanobacteria in Daqing wetlands will be clarified and the effect of environmental factors on diversity of cyanophages and cyanobacteria in Daqing wetlands will be dicovered. It provide important information for investigating the relationship between cyanophages and cyanobacteria, exposing the community structure and function of cyanophage in wetland ecosystem and developing the microbial resource to control water bloom.

英文关键词： Daqing Wetland；Cyanobacteria；Cyanophage；Diversity；