基于NIR-QDs的受体免标记FRET新方法及其临床血样分析

项目名称： 基于NIR-QDs的受体免标记FRET新方法及其临床血样分析

项目编号： No.21305152

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 王宇辉

作者单位： 中国科学院宁波材料技术与工程研究所

项目金额： 25万元

中文摘要： 荧光共振能量转移(FRET)是一种灵敏，均相的检测技术，但是，由于生物基质本底荧光，散射光和光吸收等干扰，其应用于血样分析时灵敏度大大降低；此外，FRET受体通常需要繁琐的修饰或标记步骤。本项目以疾病标志物为对象，设计了基于NIR-QDs修饰的aptamer/peptide为供体，无机猝灭材料(碳纳米颗粒，芳香聚合物)为免标记受体的FRET新方法。其以近红外为检测窗口(650-900 nm)，极大地消除生物样本的光学干扰，提高了信噪比；利用aptamer/peptide与纳米受体的π-π相互作用实现供受体自组装，避免近红外有机染料或纳米金为受体时繁琐的生物标记或表面修饰,合成困难，成本较高等不足，提高分析效率。本研究利用NIR-QDs优良的光学性质，纳米受体优异的猝灭能力和aptamer/peptide的高特异性来提高FRET分析性能，尤其是在临床复杂血样,对拓宽近红外分析具有重要意义。

中文关键词： 荧光共振能量转移；量子点；近红外；纳米受体；免标记

英文摘要： Fluorescence resonance energy transfer (FRET) is a highly sensitive and homogeneous assay technology. However, the assay sensitivity in blood sample will be lowered, which is mainly attributed to the autofluorescence，scattering light and light absorption arise from biological matrix. Additionally, the energy acceptors like organic dyes or gold nanoparticles in FRET usually need complicated and time-consuming biological label or surface modification. Aim at improving analytical performance of FRET assay, especially in complicated clinical blood sample, NIR-emitting Quantum dots (QDs) and nanoquencher with unique structure and electronic properties are adopted. The project is proposed to develop new FRET strategy with aptamer/peptide tagged NIR emitting QDs as the donor and inorganic nanoquencher (carbon nanoparticles, aromatic polymers) as the label-free acceptor. In the near-infrared (650 - 900 nm) detection window, the optical interference in biological sample can be completelly eliminated, and thus, the ratio of S/N(signal to noise) is enhanced. The FRET process is induced by the π?π stacking interaction between aptamer/peptide and π-electron riched nanoquencher, which dexterously avoids biological label or surface modification typically existed in the traditional energy acceptors, resulting in low-cost and s

英文关键词： Fluorescence resonance energy transfer；Quantum dots；Near infrared；Nanoacceptor；Label-free