微生物群落定向调控微观机制研究

项目名称： 微生物群落定向调控微观机制研究

项目编号： No.31300107

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 来国莉

作者单位： 北京大学

项目金额： 22万元

中文摘要： 本项目拟在长期富集培养原油降解微生物群落工作的基础上，深入系统地研究原油富集调控微生物群落结构演替规律，全面解析群落构建过程中原油组分和代谢产物、微生物群落组成和功能基因动态变化；阐明微生物群落构建过程的微观调控机制及其关键调控因子（短板微生物、短板化学物质等）与作用，构建原油降解各变量相互作用模型并验证，为研究好氧微生物驱油机理、定向调控微生物群落进行微生物采油技术的开发和应用提供理论指导和实验依据。

中文关键词： 微生物群落；驯化；宏基因组；GeoChip；

英文摘要： It is reported that a small but perfectly formed microbial community was acclimated by crude oil as the sole source of carbon and energy. Furthermore, systematic study on the dynamic changes of the composition of microbial community, the components of crude oil, degradation metabolites of crude oil, as well as the functional genes were performed. Our study was focus on the clarification the micro-regulation mechanism and the key regulatory factors, such as short board microorganisms, short board chemicals, etc., on the construction of microbial community by enrichment culture. Interaction model of each variable in the process of crude oil degradation was proposed and then verified, which will contribute to the technology application of microbial enhanced oil recovery by directional regulation of microbial community.

英文关键词： Microbial community；Acclimation ；Metagenome；GeoChip；